Neuroscience 2005 Abstract
| Presentation Number: | 484.13 |
|---|---|
| Abstract Title: | Profiling of synapse proteome function using transcriptome and electrophysiological microarrays on primary hippocampus neurons. |
| Authors: |
Valor, L. M.*1
; Collins, M. O.1
; Humphreys, L.1
; Charlesworth, P.1
; Grant, S. G. N.1
1Team 32, Genes to Cognition, Wellcome Trust Sanger Inst., Cambridge, United Kingdom |
| Primary Theme and Topics |
Development - Synaptogenesis and Activity-Dependent Development -- Synaptogenesis and activity-dependent plasticity |
| Secondary Theme and Topics | Neural Excitability, Synapses, and Glia: Cellular Mechanisms<br />- Synaptic Transmission<br />-- Postsynaptic organization and structure |
| Session: |
484. Activity-Dependent Development III Poster |
| Presentation Time: | Monday, November 14, 2005 1:00 PM-2:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # C30 |
| Keywords: | SYNAPSE, GENE EXPRESSION, ELECTROPHYSIOLOGY, CULTURE |
The process of synapse formation and assembly of neuronal circuits can be studied in vitro using primary hippocampus neuronal culture. Previous proteomic studies have characterised around 1000 proteins in the Post Synaptic Proteome (PSP) and 185 proteins in the NMDA receptor complex (NRC) (see Collins et al, SfN 2005). We have addressed the assembly of the PSP and NRC using gene expression profiles in cultured neurons. In addition, it is possible to correlate changes in gene expression with developmental changes in electrophysiological properties. For these purposes, we have combined two distinct microarray technologies: high density oligonucleotide arrays for genome wide transcriptome profiling (Murine Genome 430 2.0 array, Affymetrix) and multielectrode array technology (Multichannel Systems) for recording activity in populations of neurons. Data was collected at time points between day 1 and day 25 during which time there was a sigmoidal increase in action potential firing and emergence of complex firing patterns. Gene expression profiles showed distinct sets of synaptic proteins were co-ordinately regulated in a temporally sequential fashion. High levels of expression of key signalling proteins, including those in the NRC, preceded the increase in expression of activity-dependent genes. The combination of these array technologies provides a powerful functional genomics approach for studying neuronal physiology and gene expression, towards a new model for the assembly of the synapse proteome.
Supported by Fundacion Ramon Areces, Spain; The Wellcome Trust, UK
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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