Neuroscience 2005 Abstract
| Presentation Number: | 484.1 |
|---|---|
| Abstract Title: | Activity-dependent regulation of transcription factors and coactivator in cultured rat visual cortical neurons. |
| Authors: |
Liang, H. L.*1
; Wong-Riley, M. T-. T.1
1Dept. of Cell Biology Neurobiology, Med. Col. of Wisconsin, Milwaukee, WI |
| Primary Theme and Topics |
Sensory and Motor Systems - Vision -- Visual cortex: Functional organization and circuitry |
| Secondary Theme and Topics | Neural Excitability, Synapses, and Glia: Cellular Mechanisms<br />- Synaptic Plasticity<br />-- Other |
| Session: |
484. Activity-Dependent Development III Poster |
| Presentation Time: | Monday, November 14, 2005 1:00 PM-2:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # C30 |
| Keywords: | cytochrome oxidase, real-time quantitative PCR, tetrodotoxin, transcription factors |
Several transcription factors have been identified as important mediators of mitochondrial biogenesis, which requires coordinated regulation of both the nuclear and mitochondrial genomes. Nuclear respiratory factor 1 (NRF-1) and 2 (NRF-2) activate transcription of several respiratory chain enzymes and are prime candidates for bigenomic coordinated regulation of cytochrome oxidase (COX) subunit genes derived from the two genomes. Peroxisome proliferator-activated receptor gamma coactivator 1 (PGC-1) is a known coactivator of NRF-1 and/or NRF-2-dependent transcription. Previously, we showed that increased neuronal activity via depolarizing stimulation induced an up-regulation of NRF-2 protein in vitro, whereas monocular deprivation caused a down-regulation of NRF-2 protein and mRNA in vivo. Both changes paralleled those of COX activity. We hypothesized that the expression of NRF-1, NRF-2, and PGC-1 genes are also regulated by neuronal activity. To test this hypothesis, primary cultured neurons were exposed to tetrodotoxin (TTX) for 2, 4, and 6 days. Expressions of PGC-1, NRF-1, and NRF-2 mRNAs and proteins in neurons were compared by using real-time quantitative PCR and Western blots, respectively. Results indicated that there was significant reductions of PGC-1 mRNA after 2 days, NRF-2β after 4 days, and NRF2α as well as NRF-1 after 6 days of TTX exposure. At the protein level, PGC-1 was significantly decreased after 4 days of TTX, whereas NRF-1, NRF-2α, and NRF-2β were significantly reduced after 6 days. Our results suggest that these transcription factors and coactivators are sensitive to and are regulated by neuronal activity.
Supported by NIH EY05439
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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