Neuroscience 2004 Abstract
| Presentation Number: | 456.4 |
|---|---|
| Abstract Title: | Dendritic spine motility and length is temperature dependent and correlate with the neuroprotective effect of hypothermia against damage following OGD. |
| Authors: |
Gisselsson, L. L.*1
; Matus, A.2
; Wieloch, T.1
1Exptl. Brain Res, Lund, Sweden 2Switzerland, Lund Univ, 22184, |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia -- Neuroprotection and tolerance |
| Session: |
456. Ischemia: Neuroprotection and Tolerance V Poster |
| Presentation Time: | Monday, October 25, 2004 11:00 AM-12:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # ZZ14 |
| Keywords: | dendritic spines, plasticity, ischemia, hippocampus |
Reduction of brain temperature by a few degrees dramatically influences outcome following brain ischemia, and is still the only effective neuroprotective treatment brain ischemia in man. Several mechanisms have been proposed to contribute to the neuroprotective effect of therapeutic hypothermia, suggesting that multiple processes are involved. Receptor blockade or disruption scaffold protein-receptor interactions affect ischemic damage. Hence, the dendritic spines appear to be the prime site of action leading to cell death. Complex dynamic dendritic processes such as receptor trafficking or changes in spine morphology require cooperative processes of interaction of multiple protein-protein interactions which influence by small changes in temperature. Dendritic spines are motile structures, changing shape and length within the time span of seconds. This rapid motility is mainly due to changes in rearrangements of the cytoskeleton actin filaments. We studied the effect of temperature on spine motility using hippocampal neurons (7DIV) from transgenic actin-GFP mice. The dissociated neurons display characteristics neuronal phenotype with GFP-actin located in the spines. The motility of the spines was studied at three temperatures (27, 33, and 37°C). Also the effect of oxygen and glucose deprivation on cell death at different temperatures was studied. We found that reduction of temperature of the perfusate by 4 degrees dramatically reduces spine motility and length of the spine shaft, which correlated with the neuroprotective effect of hypothermia. We propose that the neuroprotective effect of hypothermia reside in the inhibition of processes involved in spine dynamics.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
Copyright © 2004-2025 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
