Neuroscience 2000 Abstract
| Presentation Number: | 509.6 |
|---|---|
| Abstract Title: | Control of axon guidance in <I>Drosophila melanogaster</I> by neuronal receptor tyrosine phosphatases. |
| Authors: |
Schindelholz, B.*1
; Zinn, K. G.1
1Biology 216-76, California Institute of Technology, Pasadena, CA |
| Primary Theme and Topics |
A. Development and Regeneration - 7. Axon guidance mechanisms and pathways |
| Secondary Theme and Topics | A. Development and Regeneration<br />- 6. Process outgrowth, growth cones and sprouting |
| Session: |
509. Axon guidance mechanisms and pathways VI Poster |
| Presentation Time: | Tuesday, November 7, 2000 2:00 PM-3:00 PM |
| Location: | Hall G-J |
| Keywords: | AXON**, PHOSPHATASE, ADHESION, MOTONEURON* [MOTOR NEURON] |
Cell adhesion is critical for the establishment of proper connections in the nervous system. Some receptor-type protein tyrosine phosphatases (RPTPs) have adhesion molecule-like extracellular domains that may transduce signals in response to adhesion. By sequence comparison of the extracellular and cytoplasmic domains of the known RPTPs with the DNA database of the Drosophila genome project, we found several loci which may code for new RPTPs. Screening of embryonic cDNA libraries revealed clones encoding two proteins whose genes are closely linked at position 52F. One of these, RPTP52F is a novel receptor tyrosine phosphatase (881 amino acids), that has extracellular domains consisting of multiple fibronectin type III repeats. We have not yet been able to determine the expression pattern for RPTP52F by in situ hybridization. Recently, dsRNA was found to be a potent and specific inhibitor of gene activity in drosophila melanogaster (Kennerdell and Carthew, 1998; Misquitta and Paterson, 1999). Therefore, dsRNA-mediated genetic interference (RNAi) was used to determine the gene function of RPTP52F. Our data suggest that DPTP52F is involved in axon outgrowth and guidance during development of the CNS and the neuromuscular system. We have isolated point mutations in Ptp52F. The phenotype of the DPTP52F mutant correlates with the RNAi data. At the moment we are isolating additional DPTP52F mutants and are looking at genetic interactions with the four known RPTPs.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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