Neuroscience 2004 Abstract
| Presentation Number: | 414.13 |
|---|---|
| Abstract Title: | Expression of ionotropic glutamate receptor subunits in the lobster olfactory organ. |
| Authors: |
Hollins, B.*1
; McClintock, T. S-.2
1Dept Clin. Sci., Univ Kentucky, Lexington, KY 2KY, 900 S. Limestone St., 40536-0200, |
| Primary Theme and Topics |
Sensory Systems - Chemical senses -- Olfaction: CNS pathways and physiology |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Ligand Gated Ion Channels<br />-- Glutamate receptors: Non-NMDA receptors |
| Session: |
414. Sensory Systems: Invertebrate I Poster |
| Presentation Time: | Monday, October 25, 2004 8:00 AM-9:00 AM |
| Location: | San Diego Convention Center - Hall A-H, Board # V22 |
| Keywords: | olfactory receptor neurons, crustacea, presynaptic receptors, olfaction |
In the lobster, presynaptic inhibition of odor signals is mediated by ionotropic GABA and histamine receptors (Wachoviak et al , Microsc. Res. Tech. 58:365-375, 2002). We previously isolated a cDNA clone encoding an ionotropic glutamate receptor subunit (Lobster GluR1) using representational difference analysis of the lobster olfactory organ. Here, we report the isolation of a cDNA encoding a second glutamate receptor subunit that is also expressed in the lobster olfactory organ.
Lobster olfactory organ cDNA was used to generate a plasmid library containing 120 bp inserts that were generated by PCR using degenerate primers. Selected inserts were sequenced and tested for homology using blast analysis at the NCBI web site. 5 prime and 3 prime RACE reactions were performed on one positive insert to generate the full-length clone. Expression of the clone was tested by PCR on cDNA from several lobster tissues. In situ hybridization was performed on lobster olfactory organ using 50 micron vibratome slices and dig RNA probes.
The full-length clone encodes a 813 amino acid protein and shows 26% identity to lobster GluR1 at the protein level. In situ hybridization verified expression of the subunit in olfactory receptor neurons. Qualitative PCR indicated that the subunit is also expressed in other lobster tissues. The presence of ionotropic glutamate receptor subunits in olfactory receptor neurons suggests that they comprise an autoreceptor that function in the augmentation of odor signals.
Lobster olfactory organ cDNA was used to generate a plasmid library containing 120 bp inserts that were generated by PCR using degenerate primers. Selected inserts were sequenced and tested for homology using blast analysis at the NCBI web site. 5 prime and 3 prime RACE reactions were performed on one positive insert to generate the full-length clone. Expression of the clone was tested by PCR on cDNA from several lobster tissues. In situ hybridization was performed on lobster olfactory organ using 50 micron vibratome slices and dig RNA probes.
The full-length clone encodes a 813 amino acid protein and shows 26% identity to lobster GluR1 at the protein level. In situ hybridization verified expression of the subunit in olfactory receptor neurons. Qualitative PCR indicated that the subunit is also expressed in other lobster tissues. The presence of ionotropic glutamate receptor subunits in olfactory receptor neurons suggests that they comprise an autoreceptor that function in the augmentation of odor signals.
Supported by NIH grant 5R03Dc04433-02
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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