Neuroscience 2005 Abstract
| Presentation Number: | 29.15 |
|---|---|
| Abstract Title: | Cdc2 promotes peripheral axon regeneration via the activation of Schwann cells. |
| Authors: |
Han, I.*1
; Seo, T.1,2,3
; Kim, J.1,3
; Yoon, J.2
; Yoon, S.3
; Kim, D.1
; Namgung, U.1
1Oriental Medicine, Daejeon Univ., Daejeon, Democratic People's Republic of Korea 2South Korea, 96-3 Yongun-dong, 300-716, 3Sprts and Leisure Studies, 96-3 Yongun-dong, 300-716, |
| Primary Theme and Topics |
Development - Transplantation and Regeneration -- Regeneration: PNS |
| Secondary Theme and Topics | Development<br />- Neurogenesis and Gliogenesis<br />-- Proliferation |
| Session: |
29. Regeneration: PNS II Poster |
| Presentation Time: | Saturday, November 12, 2005 3:00 PM-4:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # C6 |
| Keywords: | RAT, Sciatic nerve, PROLIFERATION, MIGRATION |
Cdc2 kinase is a prototypical cyclin-dependent kinase critical for G2 to M phase cell cycle transition. Yet, its function in the nervous system is largely unknown. Here, we investigated possible role of Cdc2 in axonal regeneration using sciatic nerve system in rat. Cdc2 protein levels and activity were increased in the injured sciatic nerves 3 and 7 days after crush injury and then decreased to basal level 14 days later. Administration of Cdc2 kinase inhibitor roscovitine in vivo at the time of crush injury significantly inhibited axonal regeneration when regrowing axons were analyzed using anterograde and retrograde tracers. Immunohistochemical analysis showed that induced Cdc2 protein was localized in the Schwann cells, and Cdc2 protein levels in cultured Schwann cells which were prepared from sciatic nerves 7 days after crush injury were much higher compared with those from uninjured sciatic nerves, suggesting that Cdc2 protein expression was primarily induced in the Schwann cells. To further investigate Cdc2 function in Schwann cell, we visualized proliferating Schwann cells in vivo by BrdU labeling method and found increased Schwann cell labeling in an actively regenerating area of the sciatic nerves. Similarly in the cultured Schwann cells, BrdU labeling was colocalized with the ones expressing Cdc2. Both the number of proliferating Schwann cells and the extent of neurite outgrowth from co-cultured DRG neurons were significantly decreased by roscovitine treatment in DRG culture which was prepared from animals with sciatic nerve injury for 7 days. Also, Schwann cell migration in the injured sciatic nerve explants was significant inhibited by roscovitine treatment. Taken together, the present data suggest that Cdc2 may be involved in peripheral nerve regeneration via Schwann cell proliferation and migration.
Supported by Korea Research Foundation (C00161)
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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