Neuroscience 2005 Abstract
| Presentation Number: | 29.11 |
|---|---|
| Abstract Title: | Electrical stimulation promotes peripheral axon regeneration by enhancing autocrine/paracrine neurotrophin signaling. |
| Authors: |
English, A. W.*1
; Mulligan, A.1
; Schwartz, G.1
1Dept Cell Biol, Emory Univ. School of Medicine, Atlanta, GA |
| Primary Theme and Topics |
Development - Transplantation and Regeneration -- Regeneration: PNS |
| Secondary Theme and Topics | Disorders of the Nervous System<br />- Trauma<br />-- Peripheral nerve |
| Session: |
29. Regeneration: PNS II Poster |
| Presentation Time: | Saturday, November 12, 2005 3:00 PM-4:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # C6 |
| Keywords: | BDNF, GRAFT, NT-4/5, trkB |
Electrical stimulation of the proximal stump of a cut peripheral nerve at the time of its surgical repair results in an enhancement of the regeneration of its axons. Stimulation also results in an increase in the mRNA for brain derived neurotrophic factor and its receptor, trkB, in motoneurons and dorsal root ganglion cells. The regeneration of axons through nerve grafts was used to evaluate whether the growth promoting effect of electrical stimulation is due to an enhancement of cell autonomous neurotrophin signaling in the regenerating axons or enhancement of signaling from neurotrophins in the environment surrounding them. Experiments were conducted using the thy-1-YFP-H mouse, in which a subset of peripheral axons is marked by the presence of yellow fluorescent protein (YFP). A single one hour application of electrical stimulation at the time of surgical repair of the cut common fibular (CF) nerve results in a doubling of the median length of profiles of regenerating YFP+ axons through nerve grafts from wild type (C57BL6/J) littermates, both one or two weeks later. Growth of regenerating axons through grafts from neurotrophin-4/5 knockout mice at these same time points is normally very poor, but electrical stimulation produces an enhancement of growth to the same extent found in grafts from wild type mice which had been stimulated. Normally weak growth of axons through grafts made acellular by repeated cycles of freezing and thawing also can be restored by electrical stimulation. Stimulation of the sciatic nerve on one side of a mouse resulted in a similar enhancement of the regeneration of CF axons on both sides of the animal in all of the treatment groups studied. Electrical stimulation thus produces a potent enhancement of the regeneration of axons in cut peripheral nerves which is independent of neurotrophin production by cells in their surrounding environment. The effects of this enhancement are more widespread than the nerve stimulated.
Supported by HD32571 from the USPHS.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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