Neuroscience 2005 Abstract
| Presentation Number: | 29.7 |
|---|---|
| Abstract Title: | Schwann cells express sensory and motor phenotypes that control axon regeneration. |
| Authors: |
Brushart, T. M.*1
; Redett, R.3
; Hameed, H.1
; Li, Z.1
; Jari, R.1
; Zhou, C.2
; Hoke, A.2
1Dept Orthopaedic Surgery, Johns Hopkins, Baltimore, MD 2Neurology, Johns Hopkins, Baltimore, MD 3Plastic Surgery, Johns Hopkins, Baltimore, MD |
| Primary Theme and Topics |
Development - Transplantation and Regeneration -- Regeneration: PNS |
| Session: |
29. Regeneration: PNS II Poster |
| Presentation Time: | Saturday, November 12, 2005 3:00 PM-4:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # C6 |
| Keywords: | NGF, BDNF, GDNF, Pleitrophin |
Schwann cell phenotype is usually described as myelinating or non-myelinating. However, we have recently shown that Schwann cells of cutaneous nerve differ substantially from those of ventral root in their growth factor expression (NSci Abst 373.2, 2004). Cutaneous nerve, denervated or reinnervated by cutaneous axons, dramatically upregulates NGF and BDNF but expresses little GDNF or Pleitrophin. Under similar conditions, ventral root upregulates GDNF and Pleitrophin 20-40 fold while expressing virtually no NGF or BDNF. Cutaneous and motor Schwann cells thus exhibit distinct phenotypes. The current experiments examine the effect of cutaneous and motor Schwann cell phenotype on cutaneous axon regeneration. Experiments were performed on the femoral nerve of Lewis rats. Two-cm-long grafts of femoral cutaneous nerve (1,600 myelinated axons) or L5 ventral root (2200 myelinated axons) were sewn to the transected femoral cutaneous nerve to be reinnervated by femoral cutaneous axons. Regeneration was assayed after 15 days of regeneration. DRG neurons that projected axons 1 cm down the graft were labeled with Fluoro Ruby by injecting a transverse crush site through a micropipette. A mean of 950 DRG neurons was labeled from cutaneous nerve grafts (n=8), while a mean of only 339 neurons was labeled from ventral root (n=8) (p=0.0001). Ventral root, even with an excess of Schwann cell tubes, is thus inferior to cutaneous nerve in its ability to support cutaneous axon regeneration. Inability to upregulate NGF or BDNF is a likely contributor to this failure. Ongoing experiments will determine the durability of sensory/motor phenotype after inappropriate reinnervation, the trophic responses to reinnervation by motor axons, and the ability of cutaneous nerve and ventral root to support motor axon regeneration.
Supported by NIH RO1 NS034484 and JHU Dept of Surgery
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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