Neuroscience 2003 Abstract
| Presentation Number: | 305.16 |
|---|---|
| Abstract Title: | Graded reoxygenation with chemical inhibition of oxidative phosphorylation improves posthypoxic recovery in murine hippocampal slices. |
| Authors: |
Huber, R.*1
; Spiegel, T.1
; Buechner, M.1
; Timmler, M.1
; Riepe, M. W.1
1Dept. Neurol, Univ. Ulm, Ulm, Germany |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia -- Neuroprotection and tolerance |
| Session: |
305. Ischemia: Cell & Molecular Mechanisms IV Poster |
| Presentation Time: | Sunday, November 9, 2003 4:00 PM-5:00 PM |
| Location: | Morial Convention Center - Hall F-I, Board # FF5 |
| Keywords: | ISCHEMIA, OXIDATIVE STRESS, STROKE, RESPIRATION |
Background and Purpose: Rapid and complete tissue reoxygenation is a prime goal of present stroke therapy. However, reoxygenation may trigger detrimental cascades that partly antagonize beneficial effects. It was the goal to investigate selective grading of reoxygenation with targeting of single mitochondrial complexes in murine hippocampal slices.
Methods: Population spike amplitude (PSAP) and NADH were measured during hypoxic hypoxia and recovery thereof. With onset of reoxygenation slices were treated for different times with amobarbital (1 mM), malonate (2 mM), or cyanide (1 mM), inhibitors of mitochondrial complex I, II, or IV, respectively. Other slices were treated with nicotinamide (1 mM).
Results: Posthypoxic recovery of PSAP increased from 32 ± 43 % of onset in control slices to 52 ± 59 % (p < 0.05) upon treatment with amobarbital for 1 minute and 62 ± 37 % (p < 0.05) upon treatment with malonate. With nicotinamide posthypoxic recovery improved up to 73 ± 25 % (p < 0.05). Oxidation of NADH was prolonged upon treatment with amobarbital while no change in NADH oxidation was observed with malonate and nicotinamide.
Conclusions: Grading reoxygenation with targeting of mitochondrial complexes I or II but not of complex IV improves outcome upon reoxygenation in murine hippocampal slices.
Methods: Population spike amplitude (PSAP) and NADH were measured during hypoxic hypoxia and recovery thereof. With onset of reoxygenation slices were treated for different times with amobarbital (1 mM), malonate (2 mM), or cyanide (1 mM), inhibitors of mitochondrial complex I, II, or IV, respectively. Other slices were treated with nicotinamide (1 mM).
Results: Posthypoxic recovery of PSAP increased from 32 ± 43 % of onset in control slices to 52 ± 59 % (p < 0.05) upon treatment with amobarbital for 1 minute and 62 ± 37 % (p < 0.05) upon treatment with malonate. With nicotinamide posthypoxic recovery improved up to 73 ± 25 % (p < 0.05). Oxidation of NADH was prolonged upon treatment with amobarbital while no change in NADH oxidation was observed with malonate and nicotinamide.
Conclusions: Grading reoxygenation with targeting of mitochondrial complexes I or II but not of complex IV improves outcome upon reoxygenation in murine hippocampal slices.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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