Neuroscience 2003 Abstract
| Presentation Number: | 214.9 |
|---|---|
| Abstract Title: | Quantification of axon loss in experimental allergic encephalomyelitis (EAE) mice. |
| Authors: |
Lankford, K. L.*1,4
; Oliver, A. R.2,3
; Ruddle, N. H.2,3
; Kocsis, J. D.1,4
1Neurol., Yale Univ., New Haven, CT 2Epid/Public Hlth., Yale Univ., New Haven, CT 3ImmunoBiol., Yale Univ., New Haven, CT 4CT, 333 Cedar St. New Haven, CT 06510, 06510, |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Demyelinating Disorders |
| Secondary Theme and Topics | Sensory Systems<br />- Anatomy |
| Session: |
214. Demyelinating Disorders: Mechanisms of Disease--EAE & PNS Poster |
| Presentation Time: | Sunday, November 9, 2003 8:00 AM-9:00 AM |
| Location: | Morial Convention Center - Hall F-I, Board # UU64 |
| Keywords: | Demyelination, Axonopathy, Multiple sclerosis, Inflammatory disease |
Although axon degeneration is believed to contribute significantly to the permanent functional deficits in diseases such as multiple sclerosis, the extreme variability in sizes and locations of lesions associated with autoimmune diseases has made it difficult to evaluate the effectiveness of interventions intended to prevent such axon loss. In this study we developed a strategy for assessing the degree of axon loss a murine EAE model based on the anatomical convergence of somatosensory sensory axons. EAE was induced in 8-10 week C57BL/6 mice by immunization with recombinant myelin oligodendrocyte glycoprotein (MOG) peptides in complete Freund’s adjuvant and pertussus toxin to enhance immunological responses (Suen et al 1997, J. Exp. Med. 186:1233-1240). Four weeks after initial immunization, mice were perfused and spinal cords processed for standard plastic sectioning. Cross-sectional areas of dorsal columns were measured in 1 µm sections collected every 0.25 mm and axons were then counted at 5 predefined positions within the dorsal column in a 2 mm segment of cord beginning 1 mm caudal to the obex. Calculations based on these data showed quantitatively significant sensory axon losses in the EAE models examined, as well as revealing localized swellings of the dorsal funiculus which are presumably due to inflammation. We plan to use this quantitative morphometric approach to evaluate interventional strategies such as cell transplantation designed to prevent axon loss.
Supported by NIH, NMSS and the Medical Res. and RR and D Services of the Dept. of Veterans Affairs.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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