Neuroscience 2005 Abstract
| Presentation Number: | 249.9 |
|---|---|
| Abstract Title: | Screen for cues involved in the axon guidance of midbrain dopaminergic neurons. |
| Authors: |
Hammond, R.*1
; Leete, T.1
; Abeliovich, A.1
1Pathology Dept, Columbia Univ., New York, NY |
| Primary Theme and Topics |
Development - Axonal and Dendritic Development -- Axon growth and guidance: Other |
| Session: |
249. Axon Growth and Guidance I Poster |
| Presentation Time: | Sunday, November 13, 2005 1:00 PM-2:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # B6 |
| Keywords: | axonal pathfinding , dopamine |
Midbrain dopaminergic neurons differentiate adjacent to the ventral floor plate at approximately E10.5 in the mouse. These neurons extend axons rostrally - away from the isthmus and towards the striatum (E11.5 - E14.5). To date there is limited data regarding the cues that may govern this projection. The dorsal portion of the neuroepithelium has been proposed as a source of guidance cues (Nakamura et al, 2000), and recently Slit2 and netrin-1 have been shown respectively to inhibit or promote midbrain dopaminergic axonal extension in vitro at E14.5 (Lin et al, 2005). However an analysis of the midbrain expression of axon guidance cues over the time period of axonal extension by this population remains lacking. We have addressed this using in situ hybridization techniques to probe for known axon guidance cues. In parallel studies we are addressing whether such cues have functional effects on midbrain dopaminergic neurons using an in vitro collagen co-culture assay where midbrain explants from E11.5 mice are cultured in apposition to cell transfected with the cue of interest. We hope that this combination of techniques will identify guidance cues involved in steering these neurons rostrally towards their striatal targets, and in future aim to utilize mutant mice to investigate the in vivo effects of cues found to have a functional effect in vitro.
Supported by Columbia University
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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