Neuroscience 2005 Abstract
| Presentation Number: | 249.7 |
|---|---|
| Abstract Title: | Characterizing the molecular mechanisms of CAM regulated axon growth and neural development. |
| Authors: |
Tapanes-Castillo, A.*1
; Bixby, J. L.1
; Lemmon, V. P.1
1Miami Project to Cure Paralysis, Miller School of Medicine, Univ. of Miami, Miami, FL |
| Primary Theme and Topics |
Development - Axonal and Dendritic Development -- Axon growth and guidance: Other |
| Session: |
249. Axon Growth and Guidance I Poster |
| Presentation Time: | Sunday, November 13, 2005 3:00 PM-4:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # B6 |
| Keywords: | neurite outgrowth, neurite, signal transduction, axon guidance |
Following spinal cord injury, CNS neurons require a permissive environment, rich in growth-promoting cues, for axonal growth, guidance, regeneration and recovery. Neuronal cell adhesion molecules (CAMs) mediate axon growth, branching, fasciculation, guidance and synapse formation. They are essential for proper development of the nervous system, including the corticospinal tract. As different CAMs have distinct structures and functions, the intracellular signaling mechanisms they employ must be distinct as well, yet these differences are poorly understood. Our objective is to elucidate the molecular mechanisms that underlie CAM regulated axon growth and branching; in particular, to characterize the mechanisms stimulated by the L1 immunoglobulin superfamily CAM and compare them to those employed by the calcium-dependent CAM, N-cadherin, and the extracellular matrix protein laminin. We are conducting a “high-content” screen to determine how approximately 500 chemical compounds, which regulate diverse intracellular signaling pathways, affect CAM-mediated neurite initiation, growth and branching. Our chemical library includes compounds from BIOMOL’s ICCB Known Bioactives Library as well as compounds that target additional signaling proteins of interest. The screen involves use of a Cellomics KineticScan Reader to perform automated measurement of neurite growth parameters from mouse cerebellar granule neurons grown on CAM and laminin substrates. Early results from this screen demonstrate that manipulating the activities of MAPKs, clathrin and other signaling proteins differentially affects various aspects of neuronal biology such as neurite initiation, growth and branching on CAM and laminin substrates. Data from this screen will provide insight into the signaling mechanisms employed by these diverse classes of CAMs. Furthermore, it may reveal novel proteins associated with these signaling pathways.
Supported by Buoniconti Fund, NIH FORE-SCI grant, State of FL
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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