Neuroscience 2001 Abstract
| Presentation Number: | 275.9 |
|---|---|
| Abstract Title: | PRESYNAPTIC PROTEIN SYNTHESIS IN AXON TERMINALS ISOLATED FROM ADULT RAT CORTEX. |
| Authors: |
Williams, J. M.*1,2,4
; Thompson, V. L.1
; Guevremont, D.1,2
; Abraham, W. C.3
; Tate , W. P.2
; Nairn, A. C.4
1Anatomy & Structural Biology, University of Otago, Dunedin, New Zealand 2Biochemistry, University of Otago, Dunedin, New Zealand 3Psychology, University of Otago, Dunedin, New Zealand 4Rockefeller University, New York, NY |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Synaptic Plasticity -- Long-term potentiation (LTP) |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Synaptic Transmission<br />-- Presynaptic mechanisms |
| Session: |
275. Synaptic plasticity: long-term potentiation III Poster |
| Presentation Time: | Monday, November 12, 2001 8:00 AM-9:00 AM |
| Location: | Exhibit Hall G-41 |
| Keywords: | synaptosome, elongation factor, synaptic plasticity |
Synapses can be independently modified by neural activity. Such synaptic plasticity may contribute to neural development, learning and responses to injury. Synaptic protein synthesis could account in part for rapid but persistent selective synaptic modification. Research into synaptic protein synthesis has focused on postsynaptic mechanisms. Here we report evidence for presynaptic protein synthesis. Purified axon terminals (synaptosomes) were isolated from adult rat cortex. Immunoblots demonstrated that synaptosomes were enriched for synapsin I, a synaptic marker, and negative for histone protein, a nuclear marker. Transmission electron microscopy showed the synaptosome fraction was enriched for axon terminals and contained few axons with attached dendrite. We found using RT-PCR that the synaptosomes contain beta-actin, alpha-CaMKII and elongation factor-2 mRNA but not transcription factor zif/268 mRNA. Synaptosomes were shown to undergo extramitochondrial protein synthesis by incorporation of 35S-methionine into protein in the presence of chloramphenicol. Protein synthesis was constant over 60 min and inhibited by cycloheximide. Immunoblots showed synaptosomes were positive for the protein synthesis factors eIF5 and eEF2. These data provide new evidence for presynaptic protein synthesis in the adult rat cortex. Synaptic plasticity therefore may be regulated through the co-ordinated translation of both pre- and postsynaptic mRNA.
Supported by Health Research Council of NZ and Neurological Foundation of NZ, HFSPO
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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