Neuroscience 2004 Abstract
| Presentation Number: | 196.4 |
|---|---|
| Abstract Title: | Differential innervation of prefrontal cortex by cholinergic, GABAergic, and glutamatergic basalocortical projections. |
| Authors: |
Henny, P.*1
; Jones, B. E.1
1Neurol. and Neurosurgery, Montreal Neurol Inst, MONTREAL, Canada |
| Primary Theme and Topics |
Homeostatic and Neuroendocrine Systems - Biological Rhythms and Sleep -- Sleep |
| Session: |
196. CNS State and Oscillatory Activity Poster |
| Presentation Time: | Sunday, October 24, 2004 11:00 AM-12:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # EE8 |
| Keywords: | SLEEP, BASAL FOREBRAIN, BDA, WAKING |
Through projections to the cerebral cortex, the basal forebrain plays a critical role in cortical activation and attention, yet also in cortical slow wave activity and sleep. The basalocortical projections have recently been shown to be comprised by cholinergic, GABAergic and glutamatergic fibers (Henny et al, SFN, 2002), which could be differentially involved in these opposing roles. In the present study, we examined the relative contributions of each fiber system to innervation of the limbic prefrontal cortex in the rat. For this purpose, we employed anterograde transport of biotin dextran amine (BDA) from neurons in the magnocellular preoptic-substantia innominata region with dual staining for BDA and the vesicular transporter proteins for acetylcholine (VAChT), GABA (VGAT) or glutamate (VGluT2). Based upon strereological estimates, we found that the VAChT+, VGAT+ and VGluT2+ varicosities accounted respectively for ~50%, ~40% and <10% of the total number of BDA+ varicosities in the orbitofrontal cortex. The different densities were associated with morphological differences in fibers and varicosities. Moreover, each projection differed in its relationship to calbindin- or parvalbumin-immunostained interneurons on the one hand and nonphosphorylated neurofilament (SMI-32)-immunostained pyramidal cells on the other. These results suggest that the cholinergic, GABAergic and glutamatergic basalocortical projections might contribute differently to the modulaction of cortical activity across sleep-wake states.
Supported by Research supported by NIH and CIHR grants.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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