Neuroscience 2004 Abstract
| Presentation Number: | 169.19 |
|---|---|
| Abstract Title: | Localization of receptors and signaling molecules involved in postsynaptic calcium dynamics of Purkinje cells. |
| Authors: |
Ivanova, T. N.*1
; Artinian, L. R.1
; Finch, E. A.1
1Cell Biol., Emory Univ, Atlanta, GA |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Synaptic Transmission -- Postsynaptic mechanisms: Excitatory |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Intracellular Signaling Pathways<br />-- Second messengers |
| Session: |
169. Postsynaptic Mechanisms I Poster |
| Presentation Time: | Sunday, October 24, 2004 10:00 AM-11:00 AM |
| Location: | San Diego Convention Center - Hall A-H, Board # M3 |
| Keywords: | IMMUNOHISTOCHEMISTRY, CEREBELLUM, RYANODINE RECEPTOR, IP3 RECEPTOR |
Synaptic and dendritic activity in Purkinje neurons generates spatially and temporally complex calcium (Ca) dynamics. Purkinje cells express high levels of many cellular constituents involved in Ca homeostasis, including IP3 receptors (IP3Rs) and ryanodine receptors (RyRs), which mediate Ca release from intracellular stores. The precise localization of these receptors is likely to be critical for determining Ca dynamics and function. Our previous physiological studies showed that Ca release by RyRs plays a substantial role in Ca signals generated by parallel fiber (PF) synaptic activity (Artinian and Finch, SFN abstract, 2003). In the present study we have used immunohistochemistry to analyze the localization of receptors, ion channels, and signaling molecules involved in Ca homeostasis in the cerebellum of rats at P16-24, the ages commonly used for electrophysiological and Ca imaging studies, and at P60. We find that IP3Rs, RyRs, and mGluRs are extensively colocalized in Purkinje cells. Although IP3Rs and RyRs are largely localized to the same endoplasmic reticulum compartments, the level of expression of these receptors is heterogeneous. The subcellular localization of IP3Rs and RyRs in Purkinje cell dendrites supports our electrophysiological and imaging studies demonstrating that both IP3Rs and RyRs contribute to postsynaptic Ca signaling and provides insight into potential interactions with other receptors and ion channels that regulate dendritic Ca dynamics.
Supported by Burroughs Wellcome Fund, Searle Scholar Program
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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