Neuroscience 2001 Abstract
| Presentation Number: | 211.6 |
|---|---|
| Abstract Title: | INTRINSIC AXON REPAIR FOLLOWING TRAUMATIC OPTIC NERVE INJURY IN ADULT RATS. |
| Authors: |
Hanke, J.*1
; Rohatgi, T.1
; Arndt, M.2
; Rousseau, V.1
; Lendeckel, U.2
; Ansorge, S.2
; Sabel, B. A.1
1Inst Med Psych, Otto-von-Guericke Univ, Magdeburg, Germany 2Inst Exp Med, Otto-von-Guericke Univ, Magdeburg, Germany |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Trauma -- Brain |
| Secondary Theme and Topics | Sensory Systems<br />- Vision<br />-- Retina and photoreceptors |
| Session: |
211. Trauma: brain injury--treatment II Poster |
| Presentation Time: | Sunday, November 11, 2001 2:00 PM-3:00 PM |
| Location: | Exhibit Hall XX-47 |
| Keywords: | RETINAL GANGLION CELL, AXONAL TRANSPORT, CYTOSKELETON, NEUROFILAMENT |
About 15% of retinal ganglion cells survive diffuse axonal crush of the optic nerve (ONC) in adult rats. Initially rats are blind, but vision recovers within 3 weeks. To study the basis of this recovery, we investigated recovery of anterograde and retrograde axonal transport, the restitution of the cytoskeletal organization, the time course of the calcium-influx into the retinal ganglion cells (RGCs), and the expression of axon-skeletal elements.
In axons surviving injury the anterograde transport of MiniRuby recovers distal to the crush site and derrangements of heavy neurofilaments are restored after 3 weeks. Using the method of in vivo confocal neuroimaging we also visualized retrograde transport of RGCs in the living rat and found, following injections of the fluorescence marker Yellow Green into the superior colliculus, recovery 3 weeks after ONC. Using the calcium marker Oregon Green BAPTA we showed that high amounts of intracellular calcium in dying RGCs correlated in time with increased levels of mRNA for cell death associated protein interleukin converting enzyme. At day 7 a moderate intracellular calcium increase was also detected in surviving RGCs and the amount of mRNA of the axon-transport-protein beta-actin increased as measured by quantitative RT-PCR.
Because these results can not be explained by true axon regeneration, this provides converging evidence that intrinsic axon repair occurs soon after diffuse axonal injury. We propose that this provides the neurobiological basis of recovery of vision following injury.
In axons surviving injury the anterograde transport of MiniRuby recovers distal to the crush site and derrangements of heavy neurofilaments are restored after 3 weeks. Using the method of in vivo confocal neuroimaging we also visualized retrograde transport of RGCs in the living rat and found, following injections of the fluorescence marker Yellow Green into the superior colliculus, recovery 3 weeks after ONC. Using the calcium marker Oregon Green BAPTA we showed that high amounts of intracellular calcium in dying RGCs correlated in time with increased levels of mRNA for cell death associated protein interleukin converting enzyme. At day 7 a moderate intracellular calcium increase was also detected in surviving RGCs and the amount of mRNA of the axon-transport-protein beta-actin increased as measured by quantitative RT-PCR.
Because these results can not be explained by true axon regeneration, this provides converging evidence that intrinsic axon repair occurs soon after diffuse axonal injury. We propose that this provides the neurobiological basis of recovery of vision following injury.
Supported by the BMBF Neurotraumaverbund A2 and BMBF Neuroverbundprojekt Magdeburg B4/B5.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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