Neuroscience 2003 Abstract
| Presentation Number: | 144.14 |
|---|---|
| Abstract Title: | The effects of topography on axon and dendrite growth and synapse distribution. |
| Authors: |
Dowell-Mesfin, N. M.*1,3
; Abdul-Karim, M.2
; Hussain, R. J.3
; Badri, R.2
; Turner, J. N.1,3
; Shain, W.1,3
1Dept. of Biomed. Sci, Univ. at Albany, Albany, NY 2NY, Sch of Public Hlth., 12201, 3USA, Sch of Public Hlth., 12201, |
| Primary Theme and Topics |
Development - Axonal and Dendritic Development -- Dendritic growth and branching |
| Secondary Theme and Topics | Development<br />- Synaptogenesis and Activity-Dependent Development<br />-- Synapse formation: CNS |
| Session: |
144. Dendritic Growth & Branching II Poster |
| Presentation Time: | Sunday, November 9, 2003 9:00 AM-10:00 AM |
| Location: | Morial Convention Center - Hall F-I, Board # B80 |
| Keywords: | hippocampal neurons, axon guidance, neuronal differentiation, cell culture |
Topographic cues are important for the orientation and growth of hippocampal neurons. Previously, we demonstrated that topographic signals could influence axon and dendrite orientation and extension at 14 DIV. The effects of topography on the growth and differentiation of dendrites and on synapse distribution were studied with time in culture. Topographical cues were presented as pillared arrays (widths = 0.5 and 2 µm; interpillar gaps = 1 - 5 µm) fabricated into silicon surfaces using standard photolithographic techniques and molded polystyrene replicas. Hippocampal neurons isolated from embryonic day 18 rats were seeded onto polylysine-treated surfaces and examined for as long as 21 DIV. Immunocytochemical detection of MAP-2 and βIII-tubulin-labeled processes was used to identify axons and dendrites. Images were imported into an automated 2-D tracing program to quantitatively describe process growth. Scanning electron microscopy (SEM) provided increased resolution for descriptions of neuronal growth relative to pillars, growth cone morphology, and process formation and bundling. Fluorescence microscopy demonstrated that the topography affects the distribution of synaptic proteins. At 14 DIV, synaptophysin and glutamate receptor-1 (GluR-1) have punctate patterning corresponding to the organization of pillars. Correlative light microscopy and SEM will describe more precisely synaptic protein distributions relative to the pillars. These data clearly indicate that physical cues have profound effects on neuron growth and differentiation, thus indicating that patterned topographic cues may provide additional means to establish defined patterns for neuron growth in vitro and provide a means to study the integration of individual neurons into neural networks.
Supported by NSF ECS-9876771, NIH, NIBIB, EB-000359,and NSF EEC-9986821
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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