Neuroscience 2000 Abstract
| Presentation Number: | 20.19 |
|---|---|
| Abstract Title: | Possible reason to acetylate carnosine in heart and brain--new prime of modern neurochemistry. |
| Authors: |
Boldyrev, A.*1,2
; Bulygina, E.1
; Fedorova, T.2
; Kramarenko, G.2
; Leinsoo, T.1
; Tyulina, O.1
; Oyama, Y.3
1Moscow State University , 119899 Moscow, Russian Federation 2Institute of Neurology, 123367 Moscow, Russian Federation 3Tokushima University, 770 Tokushima, Japan |
| Primary Theme and Topics |
K. Other - 149. History of neuroscience |
| Session: |
20. History of neuroscience Poster |
| Presentation Time: | Sunday, November 5, 2000 10:00 AM-11:00 AM |
| Location: | Hall G-J |
| Keywords: | antioxidant efficiency, oxidative stress, neuropeptide metabolism , histidine dipeptides |
Neuropeptide carnosine is exclusively accumulated in excitable tissues of vertebrataes, which is explained by its ability to scavenge free radicals. In heart and brain (tissues especially adopted for high level of oxygen consumption) from 30% (brain) to 95% (heart) of carnosine is present in N-acetylated form. We have compared an ability of both compounds to prevent oxidation of human serum lipoproteins, to decrease damaging effect of hypochlorite on rabbit erythrocytes, and to protect eucaryotic DNA against OH-radical breakage. In these in vitro models, N-acetylcarnosine (0.5-2.5 mM) was less effective than carnosine itself. However, in the in vivo hypobaric hypoxia test N-acetylcarnosine (100 mg/kg body weight), injected intraperitoneally to adult Wistar rats 1 hr before hypobaric hypoxia, protected animals from oxidative damage even better than carnosine. The viability of control, carnosine, and N-acetylcarnosine treated animals was 71%, 83% and 86%, respectively. Better in vivo effect of N-acetylcarnosine, being in vitro slower antioxidant than its precursor carnosine, can be explained by its higher stability against hydrolysis by serum carnosinase. If the rate of carnosine hydrolysis is taken for 100%, that of N-acetylcarnosine is only 0.3%, which is really negligible. To summarize, modern sight on biological functions of carnosine related compounds like N-acetylcarnosine has to consider both their biological activity and stability against metabolic degradation in the body.
Supported by Russian Foundation for Basic Research (# 99-04-49420)
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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