Neuroscience 2003 Abstract
| Presentation Number: | 783.4 |
|---|---|
| Abstract Title: | Stop and go traffic in the axon: relationship to synapse formation. |
| Authors: |
Sabo, S. L.*1
; Gomes, R. A.1
; McAllister, A. K.1
1Ctr. for Neurosci., Univ. California Davis, Davis, CA |
| Primary Theme and Topics |
Development - Synaptogenesis and Activity-Dependent Development -- Synapse formation: CNS |
| Session: |
783. CNS Synapse Formation II Poster |
| Presentation Time: | Wednesday, November 12, 2003 11:00 AM-12:00 PM |
| Location: | Morial Convention Center - Hall F-I, Board # A53 |
| Keywords: | SYNAPTOGENESIS, AXONAL TRANSPORT, SYNAPTIC VESICLES, CORTEX |
How synaptic components are deposited at the right place at the right time is unknown. During transport of presynaptic precursors along the developing axon, vesicles pause frequently. Here we test the hypothesis that during synapse formation, delivery of presynaptic proteins to sites of contact is mechanistically similar to the stopping seen during axonal transport. We use primary cultures of postnatal rodent visual cortical neurons and time-lapse confocal imaging of fluorescently-tagged fusion proteins to test this hypothesis. We characterize the spatial distribution of pausing in the axon. Quantitative analysis of the probability of stopping and distributions of stop durations will be presented (n=1075). We then investigate the relationship between pausing and cycling of the transport vesicles with the plasma membrane. We find that synapsins regulate pausing (n=892). Finally, we determine whether stopping is regulated by calcium and phosphorylation pathways (n>5000). We then discuss our results in terms of two models of presynaptic terminal formation: the recruitment model and the chance-encounter model.
Supported by the Alfred P. Sloan Foundation (A.K.M.), the Pew Charitable Trusts (A.K.M.), the March of Dimes (A.K.M.), NEI (A.K.M.), NINDS (S.L.S.) and NIMH (S.L.S.).
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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