Neuroscience 2003 Abstract
| Presentation Number: | 34.20 |
|---|---|
| Abstract Title: | Neogenin has an axon guidance role in the embryonic vertebrate forebrain. |
| Authors: |
Wilson, N.*1
; Cooper, H.1
; Key, B.1
1Anat. & Developmental Biol., Univ. of Queensland, Brisbane, Australia |
| Primary Theme and Topics |
Development - Axonal and Dendritic Development -- Axon growth and guidance: receptors and signaling mechanisms |
| Session: |
34. Axon Growth and Guidance: Receptors and Signaling Mechanisms--Intracellular Signaling Poster |
| Presentation Time: | Saturday, November 8, 2003 4:00 PM-5:00 PM |
| Location: | Morial Convention Center - Hall F-I, Board # B44 |
| Keywords: | NETRIN, RECEPTOR, DEVELOPMENT, XENOPUS |
Receptors for the Netrin family of axon guidance ligands in vertebrates include Deleted in Colorectal Cancer (DCC) and Neogenin. These receptors are members of a subgroup of the immunoglobulin superfamily of transmembrane proteins, and both possess four immunoglobulin-like domains, six fibronectin-type III repeats, a single transmembrane region and an intracellular region of ~300 amino acids. Although DCC and its invertebrate homologues have been shown to function in axon guidance as receptors of Netrin, a possible neural function of vertebrate Neogenin has not been elucidated. In the present study, we have examined the expression and role of Neogenin in the embryonic forebrain of Xenopus. In situ hybridisation on Stage 32 Xenopus forebrains revealed that Neogenin mRNA is specifically expressed in tissue surrounding the early axon scaffold. When Neogenin was overexpressed after blastomere injection of synthetic RNA transcripts, forebrains displayed abnormalities in the development of particular axon tracts, aberrant extension of axons into the dorsal forebrain, and the formation of ectopic neurons. Loss-of-function embryos, in which blastomeres were injected with dominant-negative Neogenin RNA transcripts, displayed axon pathfinding defects and the formation of ectopic neurons. These results demonstrate for the first time that Neogenin has a role in axon guidance in the embryonic vertebrate forebrain.
Supported by NHMRC
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
Copyright © 2003-2025 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
