Neuroscience 2001 Abstract
| Presentation Number: | 797.12 |
|---|---|
| Abstract Title: | Development of axon pathways in mice lacking normal L1 adhesion. |
| Authors: |
Itoh, K.*1
; Fushiki, S.2
; Kamiguchi, H.3
; Herrup, K.1,4
; Arnold, B.5
; Altevogt, P.5
; Lemmon, V.1
1Neurosci, Case Western Reserve Univ., Cleveland, OH 2Path & Applied Neurobiol, Kyoto Prefect. Univ., Kyoto, Japan 3Dev. Brain Science, RIKEN Brain Science Inst, Wako, Japan 4Alzheimer's Ctr, Case Western Reserve Univ., Cleveland, OH 5Tumor Immunology, German Cancer Research Center, Heidelberg, Germany |
| Primary Theme and Topics |
Development - Axonal and Dendritic Development -- Axon guidance: receptors and signaling mechanisms |
| Session: |
797. Axonal and dendritic development: axon guidance--CNS II Poster |
| Presentation Time: | Wednesday, November 14, 2001 4:00 PM-5:00 PM |
| Location: | Exhibit Hall B-62 |
| Keywords: | CAM, AXON GUIDANCE, ADHESION |
The neuronal CAM L1 is important in cell migration, axon growth and guidance. L1KO mice have defects in brain development, including hydrocephalus, abnormal corticospinal tract (CST) and hypoplasia of the cerebellar vermis. We studied mice in which the 6th Ig domain of L1 was deleted (6DKO), eliminating L1 homophilic binding. We studied the CST and the thalamocortical tract by injecting DiI crystals into the motor cortex or ventral thalamic nuclei. Both tracts showed normal development and axonal fasciculation. Histological studies and immunohistochemical analyses of neurofilament, MAP2, calbindin, and L1 cytoplasmic domain were performed. The brains from 6DKO mice showed normal anatomy without hydrocephalus or midline malformations. Other pathways including corpus callosum, anterior and hippocampal communicating fibers, striosome of the basal ganglia, and the spinal cord appeared normal. The cerebral cortex, basal ganglia, thalamus, brain stem and spinal cord showed normal cytoarchitecture. The results show that axonal guidance and neuronal migration can develop normally without the 6th Ig domain of L1. The 6th Ig domain of L1 is essential for interactions between L1 and several of its ligands previously thought to be critical for L1 function. These results suggest that other aspects of L1 function, possibly interactions with neuropilin or cytoplasmic proteins play more critical roles in neural development than L1 homophilic binding.
Supported by NEI, NICHD & RIKEN
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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