Neuroscience 2005 Abstract
| Presentation Number: | 674.2 |
|---|---|
| Abstract Title: | Genetic intervention with plasmid transfection of primary brain cultures from cynomolgus monkey. |
| Authors: |
Yasumoto, F.*1,2
; Negishi, T.2
; Kamiya, A.
; Kyuwa, S.1
; Yoshikawa, Y.1
; Sawa, A.
1Dept of Biomed Sci, Univ. of Tokyo, Tokyo, Japan 2Japan, 1-1-1 Yayoi,, 113-8657, |
| Primary Theme and Topics |
Disorders of the Nervous System - Cognitive, Emotional and Behavioral State Disorders -- Schizophrenia: Pathology |
| Session: |
674. Pathology of Schizophrenia: Genes and Expression I Poster |
| Presentation Time: | Tuesday, November 15, 2005 9:00 AM-10:00 AM |
| Location: | Washington Convention Center - Hall A-C, Board # TT26 |
| Keywords: | primate, cerebral cortex, schizophrenia |
We previously reported that some genes associated with major mental illnesses, such as DISC1 and G72, showed low sequence conservation between rodents and humans (Bord et al, SFN abstract, 2004), suggesting a possible limitation in using rodent tissues to address molecular mechanisms in the pathophysiology of mental conditions. To overcome this potential limitation, we have explored the use of primate derived primary brain cultures. We established primary brain cultures that contain neurons, astrocytes, and microglial cells from cryopreserved fetal cerebral cortex of cynomolgus monkeys (Macaca fascicularis) (Negishi et al, J Neurosci Methods, 2003). The study has faithfully followed the Guidelines for Animal Experimentation, The University of Tokyo. Here we report optimized conditions of gene transfection (expression constructs, RNAi constructs) that lead to preferential transfection to neurons or astrocytes. Application of this technique to studies of DISC1 and G72 is now shedding light on the molecular mechanisms of these genes in primate tissues.
Supported by NIH, Stanley, NARSAD, S-R, and Grants-in-Aid for Scientific Research from the Ministry of Health, Labor, and Welfare of Japan
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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