Neuroscience 2001 Abstract
| Presentation Number: | 728.11 |
|---|---|
| Abstract Title: | Quantitative analysis of axon arborization of interneurons in the rat frontal cortex. |
| Authors: |
Karube, F.*1
; Kubota, Y.1
; Kawaguchi, Y.1,2
1Lab for Neural Circuits, RIKEN, Nagoya, Japan 2Div of Cerebral Circuitry, NIPS, Okazaki, Japan |
| Primary Theme and Topics |
Motor Systems - Cortex and Thalamus -- Anatomy |
| Secondary Theme and Topics | Sensory Systems<br />- Anatomy |
| Session: |
728. Cortex and thalamus: connections of prefrontal cortex Poster |
| Presentation Time: | Wednesday, November 14, 2001 10:00 AM-11:00 AM |
| Location: | Exhibit Hall AA-3 |
| Keywords: | GABAERGIC, MORPHOMETRY, SHOLL ANALYSIS, NON PYRAMIDAL CELL |
In the rat frontal cortex GABAergic interneurons are divided into several classes based on the intrinsic firing pattern: fast-spiking (FS) cells, late-spiking (LS) cells and other types (non-FS cells). To reveal morphometric characteristics of cortical interneurons, we reconstructed axonal arborizations of physiologically identified interneuron subtypes in the frontal cortex by Neurolucida, and measured morphological parameters for axonal arbors such as total length, number of branches, mean branch length, number of orders and number of boutons. FS cells showed larger total length and more frequent branching than other types. By Sholl analysis, we quantified the spatial distribution of axonal arbors. Axonal arbors of FS and LS cells distributed more densely near the soma. Non-FS cells with descending axonal arbors were less dense in arborization near the soma and extended the axon collaterals farther. The number of boutons per unit axon length was almost same in all cell types (about 0.2 bouton/µm). By principal component (PC) analysis, we obtained four PCs (eigenvalues >1; cumulative proportion >90%). The axonal distribution around the soma and bouton distribution mainly contributed to the first PC. The second PC corresponded to the branching frequency and distance from the the soma to the farthest point of axonal arbors. Plotting cells on PC1-PC2 plane revealed several cell clusters corresponding to FS cells, LS cells and others. These results suggest that each physiological subtype of cortical interneurons show characteristic spatial distributions of axonal arbors, represented by combination of several morphometric parameters.
Supported by the FRP, RIKEN and Monbusho
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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