Neuroscience 2005 Abstract
| Presentation Number: | 603.19 |
|---|---|
| Abstract Title: | A novel method for targeted, selective, axon sparing, demyelination, and targeted cell ablation. |
| Authors: |
Qu, Y.*1
; Dilmanian, A.2
; Liu, S.1
; Rosenzwerg, E.1
; Steidinger, T.1
; Vadivelu, S.2
; Gilbert, J.2
; McDonald, J. W-.1
1International Center for Spinal Cord Injury, Kennedy Krieger Inst., Baltimore, MD 2NY, 707 N Broadway, 21205, |
| Primary Theme and Topics |
Development - Development of Motor Systems |
| Secondary Theme and Topics | Development<br />- Neurogenesis and Gliogenesis<br />-- Glial differentiation |
| Session: |
603. Development of Motor Systems II Poster |
| Presentation Time: | Tuesday, November 15, 2005 10:00 AM-11:00 AM |
| Location: | Washington Convention Center - Hall A-C, Board # D13 |
| Keywords: | REMYELINATION, GRAY MATTER, WHITE MATTER, IRRADIATION |
Current CNS injury models for studying of remyelination are associated with substantial collateral tissue damage. Here we demonstrate synchrotron-generated, high dosage x-ray microplanar beams (microbeams) as a novel tool for producing targeted and
selective demyelination. Spinal cords from rats were transaxially irradiated using 270 µM beams with incident doses ranging from 500-2000 Gy. At doses ranging from 1000-1250 Gy, immunohistochemical and electron microscope data revealed significant demyelination in white matter with minimal axon damage at three months following exposure. We also observed ablation of neurons in segmental gray matter in the microbeam path. Substantial cellular and tissue recovery occurred in white matter. In contrast, we observed an irreversible loss of axons and neurons in gray matter. In general, axonal demyelination was more prevalent in gray matter than white matter and amongst axons of small diameter than large diameter. We observed complete sparing of tissue immediately peripheral to the microbeam path. Microbeam irradiation is a novel tool for inducing targeted and selective demyelination and cellular ablation while avoid complications from tissue damage which can complicate analysis aimed at studying axonal demyelination.
selective demyelination. Spinal cords from rats were transaxially irradiated using 270 µM beams with incident doses ranging from 500-2000 Gy. At doses ranging from 1000-1250 Gy, immunohistochemical and electron microscope data revealed significant demyelination in white matter with minimal axon damage at three months following exposure. We also observed ablation of neurons in segmental gray matter in the microbeam path. Substantial cellular and tissue recovery occurred in white matter. In contrast, we observed an irreversible loss of axons and neurons in gray matter. In general, axonal demyelination was more prevalent in gray matter than white matter and amongst axons of small diameter than large diameter. We observed complete sparing of tissue immediately peripheral to the microbeam path. Microbeam irradiation is a novel tool for inducing targeted and selective demyelination and cellular ablation while avoid complications from tissue damage which can complicate analysis aimed at studying axonal demyelination.
Supported by NIH grants NS37927, NS40520, NS39577
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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