The interaction between neocortex and hippocampus is essential for encoding, storage and retrieval of memory. Input from the neocortex reaches the hippocampus (HPC) mainly by way of superficial layers of the entorhinal cortex (EC). Putative excitatory cells in these layers have multipeaked multidirectional firing fields, collectively signalling the rat’s location as efficiently as downstream place cells in the HPC (Fyhn et al., SfN 2003). We asked whether spatial location is expressed similarly in deeper layers of the EC, which receive a dense innervation back from the HPC but only sparse input from superficial layers of the EC. Tetrodes were implanted in layers V and VI of the rat EC, and spatial modulation of firing was recorded from parallel-recorded cells while the rats chased food in square (A) and circular (B) enclosures, using an A-B-A design. Putative excitatory cells in layers V and VI had stable multipeaked and multidirectional place fields similar to those observed in layers II and III. Multipea...