The domestic dog’s olfactory sense is widely recognized as being highly sensitive with a diverse function, however, little is known about the structure of their olfactory system. This study examined a cohort of mixed sex mesaticephalic canines and used Diffusion MRI (DTI), to map connections from the olfactory bulb to other cortical regions of the brain. The results were validated using the Klingler dissection method. An extensive pathway composed of five white matter tracts connecting to the occipital lobe, cortical spinal tract, limbic system, piriform lobe and entorhinal pathway was identified. This is the first documentation of a direct connection between the olfactory bulb and occipital lobe in any species and is a step towards further understanding how the dog integrates olfactory stimuli in their cognitive function. Significance Statement The highly sensitive olfactory system of the domestic dog is largely unexplored. We applied diffusion tractography and dissection techniques to evaluate the whi...