You can play a critical role in advancing funding for basic and biomedical research.

You can play a critical role in advancing funding for basic and biomedical research.

You can play a critical role in advancing funding for basic and biomedical research.

You can play a critical role in advancing funding for basic and biomedical research.

You can play a critical role in advancing funding for basic and biomedical research.

You can play a critical role in advancing funding for basic and biomedical research.

Fluorescence microscopy enables researchers to observe biological targets within both cultured cells and animal tissue specimens. Its utility is generally limited to cultured cell monolayers and 5-10 micron (μm) thick sections, as thicker samples begin ...

The consequences of aging can vary dramatically between different brain regions and cell types. In the ventral midbrain, dopaminergic neurons develop physiological deficits with normal aging that likely convey susceptibility to neurodegeneration. While nearby GABAergic neurons are thought to be more resilient, decreased GABA signaling in other areas nonetheless correlates with age-related cognitive decline and the development of degenerative diseases. Here, we used two novel cell type-specific Translating Ribosome Affinity Purification models to elucidate the impact of healthy brain aging on the molecular profiles of dopamine and GABA neurons in the ventral midbrain. By analyzing differential gene expression from young adult (7-10 month) and old (21-24 month) mice, we detected commonalities in the aging process in both neuronal types, including increased inflammatory responses and upregulation of pro-survival pathways. Both cell types also showed downregulation of genes involved in synaptic connectivity an...

We have documented the early embryonic development of hypothalamic neurons expressing β-endorphin (β-End), α-melanocyte stimulating hormone (αMSH), neuropeptide Y (NPY) and kisspeptin (Kiss1) in Rhesus macaques, an animal model that is very similar to humans. Neurons expressing both β-End and αMSH are the first to develop and are initially located in the lateral basal hypothalamus (LBH) as early as day 32-34 of gestation. By day 45 of gestation, these neurons have migrated into the medial basal hypothalamic (MBH) area as their final destination. NPY neurons within the ARH develop later and are first documented at day 44 of fetal life, at which time a cluster of neurons are present within ARH-MBH area. NPY neurons continued to be expressed within the ARH area at all later fetal ages analyzed. Similarly, kisspeptin neurons develop later compared to β-End, although, only a few cells are present in the ARH by day 44 of gestation, at which time kisspeptin is also expressed in the developing anterior lobe of the...

Watch on-demand the How to Make and Present A Poster for Neuroscience 2023 webinar from September 18, 2023.