Neuroscience 2000 Abstract
| Presentation Number: | 603.13 |
|---|---|
| Abstract Title: | Three dimensional digital mouse atlas using high resolution MRI. |
| Authors: |
Ruffins, S. E.*1
; Dhenain, M.1
; Jacobs, R. E.1
1Beckman Institute, Caltech, Pasadena, CA |
| Primary Theme and Topics |
A. Development and Regeneration - 20. Cerebral cortex and limbic system |
| Secondary Theme and Topics | K. Other<br />- 150. Teaching of neuroscience |
| Session: |
603. Cerebral cortex and limbic system: development and regeneration II Poster |
| Presentation Time: | Wednesday, November 8, 2000 8:00 AM-9:00 AM |
| Location: | Hall G-J |
| Keywords: | STRUCTURE**, DIGITAL, DEVELOPMENT**, GENE |
Three Dimensional Digital Mouse Atlas Using μMRI S.W. Ruffins, M. Dhenain, & R.E. Jacobs Biological Imaging Center, Beckman Institute, Caltech, Pasadena, CA 91125 Gene expression patterns, receptor domains, arrays of innervation, cell lineage patterns, and a host of other types of information in the embryo and adult take place in 3 spatial and 1 temporal dimensions. They occur within the context of the specific specimen being examined. Digital atlases provide a means to place specific data within the context of normal anatomy, analyze the information in multiple dimensions, and examine relationships between different types of information. In this work we present a prototypical digital atlas of the mouse embryo based on microscopic Magnetic Resonance Imaging (μMRI). μMRI is a qualitatively different, although complementary, imaging method to those used in constructing atlases with classical histological techniques. It is able to non-invasively and in 3 dimensions distinguish soft tissues within optically opaque specimens. The atlas presented here is composed of three different modules: unprocessed μMR images of fixed embryos aged 6.5 to 15.5dpc; an annotated atlas of the anterior portion of a 13.5dpc mouse where anatomical structures in transverse sections of the embryo have been delineated and linked to descriptive files; and a three-dimensional model of the 13.5dpc embryo. The whole embryo or specific organs may be displayed as 3D colored objects. As an example of how other types of information can be incorporated into this model, we have 'painted' in the gene expression pattern of Dlx5/Dlx6 genes involved in the regulation of forebrain development.
Supported by the NCRR and the Human Brain Project with contributions from NIMH & NIDA.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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