Neuroscience 2004 Abstract
| Presentation Number: | 458.2 |
|---|---|
| Abstract Title: | Magnetic resonance imaging allows in-vivo structual analysis of the injured spinal cord. |
| Authors: |
Masanneck, C.*1
; Wiedermann, D.3
; Mueller, H. W-.2
; Mueller, D.1
; Hoehn, M.3
; Hermanns, S.1
1Histology, NEURAXO BIOTEC, Duesseldorf, Germany 2Germany, Life Science Center Merowinger Platz 1A, 40225, 3Neurol., Life Science Center Merowinger Platz 1A, 40225, |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Trauma -- Spinal cord |
| Session: |
458. Spinal Cord Injury: Models and Concomitant Functions Poster |
| Presentation Time: | Monday, October 25, 2004 9:00 AM-10:00 AM |
| Location: | San Diego Convention Center - Hall A-H, Board # BBB7 |
| Keywords: | Spinal cord injury, Imaging, Immuncytochemistry, contrast agent |
Magnetic resonance imaging (MRI) is widely used to evaluate the level and extent of a spinal cord injury in humans. To investigate new pharmacological approaches the rat model can be expected to be the major experimental model. In both species, rat and humans, a fibrous scar forms within one week after injury after spinal cord injury (SCI), which has been shown to impede axonal regeneration in the rat model. Histologically, the fibrous scar can be detected by applying antibodies against collagen IV, whereas the glial scar is detected by staining of GFAP-positive astrocytes. The aim of the present study was to establish an in-vivo-model for monitoring scar development and to correlate MR with histological findings. The lesion can unequivocally be identified already after one day. Our therapeutic intervention to enable axonal regeneration in an experimental SCI model inhibited transiently collagenous scar formation. To investigate whether this therapeutic approach can be visualized in vivo, we applied 3D-Se imaging in treated and control animals. With this approach, we found no differences between animals with a histologically detected scar after MRI and animals where the development of a scar was prevented pharmacologically for at least 12 days after injury.
After intravenous injection of contrast agents, the fibrous scar was clearly marked by accumulation of the contrast agent, whereas in control animals there was only a slight accumulation of contrast agent. There was no accumulation of contrast agent in the glial scar. These results demontsrate that high resolution magnetic resonance imaging can be used to detect structural pathology of SCI in small animals, and that the fibrous, regeneration inhibiting, scar can be delimited by applying contrast agents.
After intravenous injection of contrast agents, the fibrous scar was clearly marked by accumulation of the contrast agent, whereas in control animals there was only a slight accumulation of contrast agent. There was no accumulation of contrast agent in the glial scar. These results demontsrate that high resolution magnetic resonance imaging can be used to detect structural pathology of SCI in small animals, and that the fibrous, regeneration inhibiting, scar can be delimited by applying contrast agents.
Supported by NEURAXO BIOTEC
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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