Neuroscience 2005 Abstract
| Presentation Number: | 454.13 |
|---|---|
| Abstract Title: | Manganese-enhanced MRI for <i>in vivo</i> assessment of damage and recovery after spinal cord injury in mice. |
| Authors: |
Klussmann, S.*1
; Stieltjes, B.1
; Krammer, P. H-.1
; Essig, M.1
; Wildemann, B.2
; Martin-Villalba, A.1
1Div. of Immunogenetics, German Cancer Research Ctr., Heidelberg, Germany 2Germany, Im Neuenheimer Feld, 280, 69120, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, Tracing, and Imaging Techniques |
| Secondary Theme and Topics | Disorders of the Nervous System<br />- Trauma<br />-- Spinal cord: Animal models and human studies |
| Session: |
454. Imaging by MRI and PET I Poster |
| Presentation Time: | Monday, November 14, 2005 8:00 AM-9:00 AM |
| Location: | Washington Convention Center - Hall A-C, Board # VV46 |
| Keywords: | spinal cord injury, MRI, Manganese, CD95 (Fas; Apo-1) |
Currently, no in vivo method is available for analyzing the integrity and functionality of the spinal cord in animal models of spinal cord injury (SCI). Damage and recovery are usually assessed by locomotor tests in the living animal or by histology post mortem. The fact that manganese ions mimic calcium and shorten the T1 relaxation time of water protons in magnetic resonance imaging (MRI) has brought them to the in vivo application. Manganese is taken up into cells resulting in a strong contrast enhancement in MRI that indicates neuronal function and activity. We show that intracerebroventricular injection of manganese chloride solution leads to uptake within the complete central nervous system. Following SCI manganese uptake in the spinal cord is reduced. Furthermore, the amount of manganese uptake correlates with the extent of damage as assessed histologically and by clinical locomotor tests. We have previously shown that inhibition of apoptosis by administration of neutralizing antibodies directed against the CD95 ligand leads to improved functional recovery and regeneration after SCI. This treatment also increases the manganese uptake in the injured spinal cord. Thus, treatment-mediated improvement of locomotor function can be visualized in vivo by MEMRI. Therefore, MEMRI is a fast and sensitive tool to analyze the functional status of the spinal cord and to monitor therapy response after injury in vivo. MEMRI can serve as an objective surrogate marker for neuronal function and clinical performance.
This work has been supported by the Christopher Reeve Paralysis Foundation.
This work has been supported by the Christopher Reeve Paralysis Foundation.
Supported by Brigitte Wildemann
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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