Neuroscience 2000 Abstract
| Presentation Number: | 416.1 |
|---|---|
| Abstract Title: | Functional magnetic resonance imaging, a useful tool to investigate spinal cord lesions and attempts to repair ascending spinal cord tracts. |
| Authors: |
Kiyotani, T.*1
; Lipson, A. C.1
; Josephson, A.1
; Klason, T.2
; Månson, S.3
; Olson, L.1
; Spenger, C.1,2
1Department of Neuroscience, Karolinska Inst, Stockholm, Sweden 2Department of Clinical Neuroscienceence, Karolinska Inst, Stockholm, Sweden 3Department of Experimental Research, Malmö University Hospital, Malmö, Sweden |
| Primary Theme and Topics |
A. Development and Regeneration - 23. Transplantation |
| Secondary Theme and Topics | A. Development and Regeneration<br />- 22. Regeneration |
| Session: |
416. Transplantation VI Poster |
| Presentation Time: | Tuesday, November 7, 2000 8:00 AM-9:00 AM |
| Location: | Hall G-J |
| Keywords: | NMR, OLFACTORY, TRANSPLANT, REGENERATION |
Functional magnetic resonance imaging (fMRI) was used to monitor spinal cord mediated activation of sensory motor cortex by measuring changes of blood oxygenation level-dependent (BOLD) contrast during electric stimulation of forepaw, hindpaw or tail. Single or multi-slice gradient echo images were collected using gradient echo fast imaging or spiral gradient imaging. Stimulation of fore- or hindpaws repeatedly lead to robust activation of contralateral sensorimotor cortex. The center of the main cortical representation was situated 3.4 mm lateral to the midline and 5 mm posterior to the rhinal fissure (prf) for the forepaw and 2.0 mm lateral to the midline and 6 mm prf for the hindpaw. Tail stimulation gave rise to an extended bilateral cortical activation, along the midline in medial frontal and cingulate gyrus 4 and 5 mm prf. Axonal regeneration in peripheral nerves has been obtained using degradable tubes made of polyglycolic acid (PGA) and collagen (Kiyotani et al., 1996 Brain Res, 740:66-74). To obtain axonal regeneration in the spinal cord, PGA-collagen tubes were implanted into dorsally hemisectioned spinal cords (TS-9) of adult rats (group A). Tubes were filled with cultured olfactory ensheathing cells, NT-3 and acidic FGF (group B). In group C, animals were not repaired after hemisection. Animals were sacrificed two weeks to 6 months after surgery. Immunohistochemistry revealed regenerated axonal fibers in the tubes in groups A and B. The behavior test (BBB scale) showed similar motor scores in all groups and each group contained animals with BBB scores of more than 18. Functional MRI during hindlimb stimulation indicated significant recovery of sensory systems in group B but not group C. Thus, fMRI provided a useful method for the investigation of sensory recovery in these experiments.
Supported by Swedish MFR, AMF, USPHS
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
Copyright © 2000-2025 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
