Neuroscience 2000 Abstract
| Presentation Number: | 327.1 |
|---|---|
| Abstract Title: | Diffusion MRI following spinal cord transplantation. |
| Authors: |
Schwartz, E. D.*1
; Tessler, A.3
; Wehrli, S.2
; Murray, M.3
; Hackney, D. B.1
1Radiology, U Penn, Philadelphia, PA 2CHOP, Philadelphia, PA 3Neurobiology & Anatomy, MCP/Hahnemann Univ, Philadelphia, PA |
| Primary Theme and Topics |
A. Development and Regeneration - 23. Transplantation |
| Secondary Theme and Topics | A. Development and Regeneration<br />- 22. Regeneration |
| Session: |
327. Transplantation II Poster |
| Presentation Time: | Monday, November 6, 2000 1:00 PM-2:00 PM |
| Location: | Hall G-J |
| Keywords: | IMAGING, NEUROTROPHIC, FIBROBLAST, REGENERATION* |
Pupose: This study was done to determine whether diffusion MRI (dMRI) can detect neuroprotective and regenerative effects of fibroblasts secreting brain derived neurotrophic factor (Fb-BDNF) in the white matter (WM) of the spinal cord following injury. Materials and Methods: Unmodified fibroblasts (Fb-UM) (N=2) or Fb-BDNF (N=2) were placed in a hemisection cavity. Sacrifice was at 1 or 8 weeks. Following perfusion fixation, dMRI was performed. Apparent diffusion coefficients (ADC's) parallel and perpendicular to the long axis of the cord were obtained in the rubrospinal tract (RST) where prior research has shown that Fb-BDNF transplants provide neuroprotection and promote axonal regeneration. Results: At 1 week, Fb-UM and Fb-BDNF ADC's in WM were similar, however abnormal as compared to control. At 8 weeks, ADC's rostral to the transplant were near normal in Fb-BDNF, while remaining abnormal in Fb-UM. The anisotropy index, abnormal in Fb-UM and Fb-BDNF at 1 week, was near normal rostral in Fb-BDNF by 8 weeks, but remained elevated in Fb-UM. Caudally, ADC's were abnormal in Fb-BDNF and Fb-UM. Differences in rostral and caudal WM may reflect transection in a descending tract. Fibers caudal to injury are separated from the cell body and undergo more severe degeneration. Fibers rostral to injury, however, maintain connections to the soma, are better preserved, and participate in regeneration. Conclusion: dMRI may be helpful in determining the severity of degeneration in WM of injured cord and may provide an indication of the efficacy of treatments intended to preserve damaged fibers, or to promote repair and regeneration.
Supported by NS24707 NS25921
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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