Neuroscience 2004 Abstract
| Presentation Number: | 240.16 |
|---|---|
| Abstract Title: | Measurement of a hypercapnia response in songbird brain (Taeniopygia guttata) with both optical and functional magnetic resonance imaging methods. |
| Authors: |
Boumans, T.*1
; Vignal, C.2
; Ramstein, S.2
; Mottin, S.2
; Verhoye, M.1
; Van Audekerke, J.1
; Van der Linden, A.1
1Bio-Imaging Lab, UA, Antwerp, Belgium 2France, Groenenborgerlaan 171, 2020, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
| Session: |
240. Imaging Techniques: fMRI, BOLD, and Other Poster |
| Presentation Time: | Sunday, October 24, 2004 11:00 AM-12:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # GGG19 |
| Keywords: | AVIAN, IN VIVO, ISOFLURANE, BLOOD OXYGENATION |
Whereas zebra finch males produce song in a more sexual context, females rather use long calls for acoustic recognition of conspecific and affinitive birds. Recognition of biologically relevant information in acoustic signals is thought to be accomplished (partly) by NCM (caudomedial neostriatum or nidopallium), a telencephalic brain region with a maximum size of 3 mm3.
In order to prepare in-vivo studies of NCM activation in response to acoustic stimuli, the hypercapnic response of NCM was explored with both optical imaging (non-invasive in-vivo broadband time-resolved spectroscopy) and functional MRI (Blood Oxygenation Level Dependent, BOLD). Subsequently, local variations in CBV and hemoglobin saturation levels obtained from optical data in songbird brain can be correlated with local BOLD signal variations which provide overall information on CBF, CBV and alterations in hemoglobin oxygenation. Female zebra finches were anesthetized with 2% isoflurane under spontaneously breathing, while they underwent three runs of a 7% CO2 challenge for 5 minutes, separated by 20 minutes of normocapnia.
During hypercapnia, there was a 1% increase of the optical signal reflecting an increased hemoglobin saturation level in the lateral part of NCM. The optical signal reflecting CBV changes indicated no significant variation. A similar hypercapnic challenge evoked a 2.6% BOLD signal increase indicating a higher blood oxygenation resulting from the overshoot of arterial supply.
For the first time, both methods were performed on zebra finches. The difference in % signal change indicates a different method sensitivity to detect for blood oxygenation variations. Both methods show great promises for in-vivo observations of acoustic recognition in the small zebra finch brain.
In order to prepare in-vivo studies of NCM activation in response to acoustic stimuli, the hypercapnic response of NCM was explored with both optical imaging (non-invasive in-vivo broadband time-resolved spectroscopy) and functional MRI (Blood Oxygenation Level Dependent, BOLD). Subsequently, local variations in CBV and hemoglobin saturation levels obtained from optical data in songbird brain can be correlated with local BOLD signal variations which provide overall information on CBF, CBV and alterations in hemoglobin oxygenation. Female zebra finches were anesthetized with 2% isoflurane under spontaneously breathing, while they underwent three runs of a 7% CO2 challenge for 5 minutes, separated by 20 minutes of normocapnia.
During hypercapnia, there was a 1% increase of the optical signal reflecting an increased hemoglobin saturation level in the lateral part of NCM. The optical signal reflecting CBV changes indicated no significant variation. A similar hypercapnic challenge evoked a 2.6% BOLD signal increase indicating a higher blood oxygenation resulting from the overshoot of arterial supply.
For the first time, both methods were performed on zebra finches. The difference in % signal change indicates a different method sensitivity to detect for blood oxygenation variations. Both methods show great promises for in-vivo observations of acoustic recognition in the small zebra finch brain.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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