Neuroscience 2001 Abstract
| Presentation Number: | 206.9 |
|---|---|
| Abstract Title: | TEMPORAL PROFILE OF ULTRASTRUCTURAL CHANGES IN CORTICAL NEURONS AT LATE PHASE OF STROKE. |
| Authors: |
Andersson, B.*1
; Chen, Z.2
; Sykova, E.3
; Bjelke, K.1
; Bjelke, B.1
1Dept. Clin Neuroscience, Karolinska Institutet, Stockholm, Sweden 2Dept. of Radiology, Peking Union Medical College Hospital, Peking, China 3Dept. of Neuroscience, 2nd Medical Faculty, Charles University, Prague, Czech Republic |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia -- Cellular and molecular mechanisms |
| Secondary Theme and Topics | Motor Systems<br />- Cortex and Thalamus<br />-- Imaging |
| Session: |
206. Ischemia: cellular and molecular mechanisms V Poster |
| Presentation Time: | Sunday, November 11, 2001 1:00 PM-2:00 PM |
| Location: | Exhibit Hall WW-21 |
| Keywords: | Apoptosis, cerebral cortex, Electron Microscopy, Ischemia |
B. Andersson1, Z. Chen1,3, E. Syková2, K. Bjelke1*, B. Bjelke1 1MR-Center, Dept. of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden. 2Dept. of Neuroscience, 2nd Medical Faculty, Charles University, Prague, Czech Republic. 3Dept. of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Beijing 100730, P.R.China.
We mimic late phase of stroke in an experimental rat model using a photochemically induced lesion which results in pan-necrotic brain tissue. By combining behavioural tests and magnetic resonance imaging (MRI) with morphological examination of the tissue under electron microscope (EM), we define the temporal profile of the cellular changes in the lesion and in the contralateral hemisphere.
Male Spraque-Dawley rats are used. After behavioural tests and MRI the brains are prepared for EM examination according to standard procedure.
At the ultrastructural level the neurons show changes such as nuclear chromatin clumping and condensation, membrane rupture and blebbing as well as cell shrinkage and condensation. These signs can be assigned to either apoptotic or necrotic cell death in different stages and are found most frequently at postlesion time 6 h on the lesioned side and at 24 h on the contralateral side.
The delayed cell death represents a potential therapeutic window for recovery, where mechanisms for improvement of the behavioural recovery can be studied.
We mimic late phase of stroke in an experimental rat model using a photochemically induced lesion which results in pan-necrotic brain tissue. By combining behavioural tests and magnetic resonance imaging (MRI) with morphological examination of the tissue under electron microscope (EM), we define the temporal profile of the cellular changes in the lesion and in the contralateral hemisphere.
Male Spraque-Dawley rats are used. After behavioural tests and MRI the brains are prepared for EM examination according to standard procedure.
At the ultrastructural level the neurons show changes such as nuclear chromatin clumping and condensation, membrane rupture and blebbing as well as cell shrinkage and condensation. These signs can be assigned to either apoptotic or necrotic cell death in different stages and are found most frequently at postlesion time 6 h on the lesioned side and at 24 h on the contralateral side.
The delayed cell death represents a potential therapeutic window for recovery, where mechanisms for improvement of the behavioural recovery can be studied.
Supported by Konung Gustaf V:s och Drottning Victorias Stiftelse; Knut och Alice Wallenbergs stiftelse.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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