Neuroscience 2004 Abstract
| Presentation Number: | 904.14 |
|---|---|
| Abstract Title: | Lipopolysaccharide-induced neuroinflammation following microinjection into the striatum of rats: An immunohistochemical and magnetic resonance imaging study. |
| Authors: |
Schmidt, W.*1
; Angenstein, F.2
; Niessen, H. G.2
; Wilhelmi, E.1
; Striggow, F.1
1KeyNeurotek AG, Magdeburg, Germany 2Germany, Leipziger Str 44, D-39120, |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Neurodegenerative Disorders -- Other |
| Secondary Theme and Topics | Neurological and Psychiatric Conditions<br />- Neurotoxicity<br />-- Toxic metabolic effects and disorders |
| Session: |
904. Neurodegeneration Mechanisms IV Poster |
| Presentation Time: | Wednesday, October 27, 2004 9:00 AM-10:00 AM |
| Location: | San Diego Convention Center - Hall A-H, Board # VV11 |
| Keywords: | INFLAMMATION, MRI, IMMUNOCYTOCHEMISTRY, LPS |
Lipopolysaccharide (LPS), a potent endotoxin, derived from the cell wall of gram-negative bacteria causes severe inflammation in the brain. Neuroinflammation is associated with several neurodegenerative diseases, including stroke, Parkinson’s disease, Alzheimer’s disease, multiple sclerosis and amyotrophic lateral sclerosis. The hallmark of brain inflammation is the activation of microglial cells that produce proinflammatory and neurotoxic factors, including cytokines and free radicals. In the present study we correlate magnetic resonance imaging (MRI) and immunohistochemistry applying different neuronal and glial markers to analyse neurodegenerative processes in a LPS-mediated model of neuroinflammation. As demonstrated by MRI, unilateral injection of LPS into the striatum of rats led to structural changes at different time points following endotoxin application. In parallel, immunfluorescence with Fluoro-Jade B showed an increased number of degenerating neurons in the striatum 24 hours, 3 and 7 days after LPS injection. Immunolaballing of the neuronal nuclear protein NeuN revealed that the number of neurons was dramatically reduced in the LPS-treated striatum compared to the contralateral control side. Neuronal cell death was accompanied by microglia activation as detected by staining with the microglial markers OX42 and isolectin B4.
In conclusion, this study demonstrates that non-invasive MRI is a highly sensitive approach to study neuronal inflammation and brain damage in vivo.
In conclusion, this study demonstrates that non-invasive MRI is a highly sensitive approach to study neuronal inflammation and brain damage in vivo.
Supported by KeyNeurotek AG
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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