Neuroscience 2005 Abstract
| Presentation Number: | 816.10 |
|---|---|
| Abstract Title: | <i>In vivo</i> MRI tracking of intravenously administered neural stem cells in the rat brain after transient middle cerebral artery occlusion. |
| Authors: |
Flexman, J. A.*1
; Cross, D. J.2,3
; Miyoshi, S.1
; Maravilla, K. R.2
; Kim, Y.1
; Stevenson, J.2
; Minoshima, S.1,2,3
1Department of Bioengineering, Univ. of Washington, Seattle, WA 2Department of Radiology, Univ. of Washington, Seattle, WA 3Washington National Primate Research Center, Univ. of Washington, Seattle, WA |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, Tracing, and Imaging Techniques |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Molecular and Genetic Techniques |
| Session: |
816. Imaging by MRI and PET III Slide |
| Presentation Time: | Wednesday, November 16, 2005 10:15 AM-10:30 AM |
| Location: | Washington Convention Center - Room 149A |
| Keywords: | STEM CELL, STROKE, IMAGING, IN VIVO |
Stem cells are a promising source of neuro-regeneration for devastating brain injuries such as stroke. Stem cells labeled with a paramagnetic tracer have been visualized in the brain using magnetic resonance imaging (MRI). Stereotaxic implantation of cells allows precise localization but it is invasive and cannot deliver stem cells to the entire stroke area. In this study, we investigated the migration of intravenously delivered neural stem cells (NSCs) to the stroke area using MRI after transient middle cerebral artery occlusion (MCAO). Rat NSCs were isolated from the fetal striatum on embryonic day 16 and labeled with Feridex, a superparamagnetic iron oxide-containing nanoparticle that decreases T2*-weighted MRI signal intensity, using hemagglutinating virus of Japan envelopes to maximize labeling efficiency. Six rats received a MCAO for 60 min, and three of these rats received an intravenous injection of labeled NSCs 48 hours after MCAO while the other three rats acted as controls. MRI was performed on anesthetized animals on days 0, 1, 2, 4, 8, 15 and 29 after MCAO, and consisted of T2*-weighted steady state free precession imaging for sensitivity to iron. Images were co-registered for each subject, and then registered to a stereotaxic atlas using NEUROSTAT. Ischemic areas were segmented for region of interest (ROI) analysis, where the mean of the ROI on the damaged hemisphere was divided by the mean of the equivalent ROI on the non-stroked hemisphere. The average percent decrease of this ratio was greater for subjects who had received labeled NSCs than for the control group for time points after two hours post NSC injection, and significantly lower at days 4 and 8 post MCAO (p<0.05). These results indicate MRI can detect early migration and accumulation of intravenously injected NSCs into the subacute stroke zone non-invasively.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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