Neuroscience 2005 Abstract
| Presentation Number: | 816.8 |
|---|---|
| Abstract Title: | Validation of diffusion tensor imaging for <i>in vivo</i> tracing of cortico-thalamic projections in the Göttingen minipig brain. |
| Authors: |
Dyrby, T. B.*1
; Lind, N. M.2
; Vejby Søgaard, L.1
; Alexander, D. C.3
; Parker, G. J. M.
; Paulson, O. B.1
; Jelsing, J.
1Danish Research Centre for Magnetic Resonance, Copenhagen Univ. Hosp., Hvidovre, Denmark 2Denmark, Kettegaard Alle 30, 2650, 3Dept. of Experimental Medicine, Kettegaard Alle 30, 2650, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, Tracing, and Imaging Techniques |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Data Analysis and Statistics |
| Session: |
816. Imaging by MRI and PET III Slide |
| Presentation Time: | Wednesday, November 16, 2005 9:45 AM-10:00 AM |
| Location: | Washington Convention Center - Room 149A |
| Keywords: | Diffusion Tensor Imaging , Manganese, MRI, Tractography |
Diffusion Tensor Imaging (DTI) enables in vivo tracking of axonal fibers in the brain and will be pivotal in studying brain connectivity in the pathological and healthy human brain. The accuracy of DTI, however, has not been exhaustively validated against actual anatomical data.
In the present study DTI was used for studying cortico-thalamic projections in the porcine brain. DTI tracings were evaluated against two gold-standards: manganese (Mn2+) -enhanced magnetic resonance imaging (MRI) and histochemical visualization of a histological tracer. At baseline, high resolution 3D T1-weighted MPRAGE scans of the brain were obtained in five young Göttingen minipigs on a 3T Siemens Trio scanner. Next, Mn2+ was stereotaxically injected into the prefrontal cortices (PFC) and uptake and transportation of Mn2+ were visualized using MRI on day two and four after injection. Mn2+ injection and projection sites were compared to the histological tracing. Finally, high-resolution DTI images from perfusion fixed brains were obtained on an experimental 4.7 Tesla Varian Inova scanner.
Detailed knowledge of the complex anatomical interconnections of the central nervous system (CNS) plays an important role in the understanding of brain regions. We demonstrated that, using DTI, it is possible to trace axonal fiber tracts connecting the PFC to the thalamus in the pig brain. Importantly, there was a good agreement between injection and projections sites of the anterograde transport of Mn2+ and the histological tracer, and between Mn2+ projections and fiber tracing using DTI.
In the present study DTI was used for studying cortico-thalamic projections in the porcine brain. DTI tracings were evaluated against two gold-standards: manganese (Mn2+) -enhanced magnetic resonance imaging (MRI) and histochemical visualization of a histological tracer. At baseline, high resolution 3D T1-weighted MPRAGE scans of the brain were obtained in five young Göttingen minipigs on a 3T Siemens Trio scanner. Next, Mn2+ was stereotaxically injected into the prefrontal cortices (PFC) and uptake and transportation of Mn2+ were visualized using MRI on day two and four after injection. Mn2+ injection and projection sites were compared to the histological tracing. Finally, high-resolution DTI images from perfusion fixed brains were obtained on an experimental 4.7 Tesla Varian Inova scanner.
Detailed knowledge of the complex anatomical interconnections of the central nervous system (CNS) plays an important role in the understanding of brain regions. We demonstrated that, using DTI, it is possible to trace axonal fiber tracts connecting the PFC to the thalamus in the pig brain. Importantly, there was a good agreement between injection and projections sites of the anterograde transport of Mn2+ and the histological tracer, and between Mn2+ projections and fiber tracing using DTI.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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