Neuroscience 2002 Abstract
| Presentation Number: | 696.3 |
|---|---|
| Abstract Title: | Tracking <i>in vivo</i> stem cell migration in a rat model of stroke by repeated MRI.<i></i> |
| Authors: |
Modo, M. M.*1
; Mellodew, K.2
; Cash, D.1
; Fraser, S. E.3
; Meade, T. J.3
; Price, J.2
; Williams, S. C. R.1
1Neuroimaging Research Group, Inst Psychiat Univ London, London, United Kingdom 2Neuroscience, Inst Psychiat Univ London, London, United Kingdom 3Beckman Institute, California Institute of Technology, Pasadena, CA |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia -- Neuroprotection and tolerance |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Staining, tracing and imaging techniques |
| Session: |
696. Ischemia: cellular and molecular mechanisms XV Poster |
| Presentation Time: | Wednesday, November 6, 2002 10:00 AM-11:00 AM |
| Location: | Hall A2-B3 Y-63 |
| Keywords: | Neural Transplantation, Grafting, MCAo, Neural Stem cells |
Fetal grafts show little migration after transplantation into the lesioned brain, whereas transplanted stem cells will migrate from the contralateral hemisphere to a focal ischaemic lesion. To track the migration of grafted cells, conditionally immortalized neural stem cells from the MHP36 cell line were labelled in vitro with the bifunctional contrast agent GRID which can be detected by magnetic resonance imaging (MRI) and fluorescent microscopy. Following 60 minutes of middle cerebral artery occlusion or sham surgery, rat brains were imaged by T1-, T2-, and proton density-weighted MRI in a 4.7T Varian imaging system to obtain pre-grafting coronal images of the stroke lesion. All animals were then transplanted contralaterally with 100,000 labelled stem cells in two deposits. The in vivo migration of grafted stem cells was assessed by scanning both transplanted ischaemic and control rats at 1, 7, and 14 days post-grafting. Fluorescent microscopy validated our in vivo images and indicated that some cells migrated away from the injection site in direction of the lesion. In two control animals little migration was observed away from the injection site. However, in one control rat some migration away from the injection tract was apparent. These results suggest that it is possible to track the migration of stem cells repeatedly in vivo by MRI. This approach will allow to further investigate how these transplants interact with the host brain to produce functional recovery.
Supported by Medical Research Council
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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