Studies in visual, auditory and somatosensory cortices have revealed that different cell types as well as neurons located in different laminae display distinct stimulus response profiles. The extent to which these layer and cell type specific distinctions generalize to gustatory cortex (GC) remains unknown. In this study, we performed extracellular recordings in adult female mice to monitor the activity of putative pyramidal and inhibitory neurons located in deep and superficial layers of GC. Awake, head-restrained mice were trained to lick different tastants (Sucrose, Salt, Citric Acid, Quinine, and Water) from a lick-spout. We found that deep layer neurons show higher baseline firing rates (FR) in GC with deep-layer inhibitory neurons displaying highest firing rates at baseline and following the stimulus. GC’s activity shows robust modulations prior to animals’ contact with tastants and this phenomenon is most prevalent in deep-layer inhibitory neurons. Furthermore, we show that licking activity strongly...