A precise sequence of axon guidance events is required for the development of the ocular motor system. Three cranial nerves grow towards, and connect with, six extraocular muscles in a stereotyped pattern, in order to control eye movements. The signalling protein alpha2-chimaerin (α2-CHN) plays a pivotal role in the formation of the ocular motor system; mutations in CHN1 , encoding α2-CHN, cause the human eye movement disorder Duane Retraction Syndrome (DRS). Our research has demonstrated that manipulation of α2-chn signalling in the zebrafish embryo leads to ocular motor axon wiring defects, although the signalling cascades regulated by α2-chn remain poorly understood. Here, we demonstrate that several cytoskeletal regulatory proteins - collapsin response mediator protein 2 (CRMP2), (encoded by the gene dpysl2) , stathmin1 and stathmin 2 - bind to α2-CHN. dpysl2, stathmin1 and especially stathmin2 are expressed by ocular motor neurons. We find that manipulation of dpysl2 and of stathmins in zebrafish larv...