Neuroscience 2002 Abstract
| Presentation Number: | 649.3 |
|---|---|
| Abstract Title: | Quantitative alternation of intracellular calcium store and establishment of oscillatory calcium response to neurotransmitter in cultured astrocyte via ERK MAP kinase cascade. |
| Authors: |
Morita, M.*1
; Moto, T.1
; Koduka, N.1
; Kudo, Y.1
1Lab Cell Neurobiol, Tokyo Univ Pharm & Life Sci, Tokyo 192-0392, Japan |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Glia -- Other |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Neurotransmitters<br />-- Glutamate |
| Session: |
649. Glia: other Poster |
| Presentation Time: | Wednesday, November 6, 2002 10:00 AM-11:00 AM |
| Location: | Hall A2-B3 E-32 |
| Keywords: | ASTROCYTE, CALCIUM, MAP KINASE, CYTOKINE |
As previously reported (Morita, M. et al, 2001 SFN abstract), Ca2+ response to neurotransmitters, such as glutamate and ATP of rat cortical type I astrocyte in culture is switched from transient to oscillatory by the treatment with growth factors (GFs), EGF and bFGF for several days, and pro-inflammatory cytokines, such as IL1β and TNFα and LPS cancel it. To elucidate the signal transduction pathway invoved, we examined the effect of kinase inhibitors. ERK MAP kinase cascade inhibitor, U0126 blocked the Ca2+ oscillation, but PD95098 and PI3 kinase inhibitors were not effective. This means that ERK MAP kinase cascade is essential for the switching and that both of MEK 1 and 2 are involved. This cascade was further investigated with anti-phospho-ERK antibody and zif268 promoter, which is an immediate early gene activated by ERK. The response of zif268 promoter to GFs was inhibited by both of U0126 and cytokine, but cytokine failed to inhibit the GFs induced phosphorylation of ERK. This indicates that cytokine inhibits the GFs induced Ca2+ oscillation by affecting the gene regulation after ERK activation. The regulation of calcium by this cascade was investigated by quantifying the intracellular Ca2+ store with Ca2+ ionophore. The total Ca2+ release of GFs treated astrocyte was significantly larger than those of astrocytes treated with U0126 or cytokine, or without GFs. These results indicate that the Ca2+ dynamics of astrocyte to neurotransmitter, especially Ca2+ oscillation was dually regulated by GFs and cytokines via the size of intracellular Ca2+ store, which is controlled by ERK MAP kinase cascade.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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