Neuroscience 2004 Abstract
| Presentation Number: | 478.4 |
|---|---|
| Abstract Title: | Detection of magnetically labelled transplanted embryonic stem cells in the rat brain by high resolution MRI <I>in vivo.</I> |
| Authors: |
Stroh, A.*1
; Faber, C.4
; Weir, K.1,3
; Lorenz, P.2
; Mueller, S.3
; Sieland, K.1
; Grune, T.5
; Jung, T.5
; Dirnagl, U.3
; Zimmer, C.
1Inst. of Radiology, Charite Univ. Hosp., Berlin, Germany 2Inst. of Pharm., Charite Univ. Hosp., Berlin, Germany 3Inst. of Neur., Charite Univ. Hosp., Berlin, Germany 4Germany, Schumannstr. 20/21, 10098, 5Exp. Physics, Schumannstr. 20/21, 10098, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
| Session: |
478. Imaging Techniques: MRI, fMRI, and PET Slide |
| Presentation Time: | Monday, October 25, 2004 1:45 PM-2:00 PM |
| Location: | San Diego Convention Center - Room 2 |
| Keywords: | Molecular Imaging, Parkinsons´s Disease, Stem Cells, MRI |
Objectives – We used high resolution MRI together with an effective magnetic labelling to asses the detection limit, localisation, and migration of transplanted embryonic stem cells (ESC) in a rat model of PD. Additionally the influence of the magnetic label on the biology of the cells was investigated.
Materials and Methods - ESC were magnetically labelled in vitro with Very Small Super-Paramagnetic Iron-Oxide-Particles (VSOP). The level of oxidative stress was detected by measuring the level of malonyldialdehyde (MDA). Minimal numbers of cells were transplanted into the striatum of healthy rats and MRI at 17.5 T was conducted. MRI at 7T was performed after transplantation of 1 × 105 magnetically labelled cells into the striatum of 6-OHDA lesioned rats.
Results - Incubation of ESC with VSOP leads to a highly significant uptake of iron. However the incubation of cells with iron-oxide-particles also results in a transient augmentation of oxidative stress. The MR detection limit in vivo at 17.5 T ranges below 100 ESC. After transplantation of ESC in the rat model of PD at 7T, characteristic signal extinctions in T2* weighted images were observed. Eight weeks after transplantation the hypointense signal shows a much more widespread distribution around the transplantation site.
Conclusion - Magnetic labelling of ESC is a feasible tool for the monitoring of cell-based therapies. Even if the labelling does increase cellular stress, the long term survival is not significantly affected. The number of detectable cells, less than 100, gives new possibilities for cellular MRI. Cell migration has shown to be an important issue concerning transplantation in PD.
Materials and Methods - ESC were magnetically labelled in vitro with Very Small Super-Paramagnetic Iron-Oxide-Particles (VSOP). The level of oxidative stress was detected by measuring the level of malonyldialdehyde (MDA). Minimal numbers of cells were transplanted into the striatum of healthy rats and MRI at 17.5 T was conducted. MRI at 7T was performed after transplantation of 1 × 105 magnetically labelled cells into the striatum of 6-OHDA lesioned rats.
Results - Incubation of ESC with VSOP leads to a highly significant uptake of iron. However the incubation of cells with iron-oxide-particles also results in a transient augmentation of oxidative stress. The MR detection limit in vivo at 17.5 T ranges below 100 ESC. After transplantation of ESC in the rat model of PD at 7T, characteristic signal extinctions in T2* weighted images were observed. Eight weeks after transplantation the hypointense signal shows a much more widespread distribution around the transplantation site.
Conclusion - Magnetic labelling of ESC is a feasible tool for the monitoring of cell-based therapies. Even if the labelling does increase cellular stress, the long term survival is not significantly affected. The number of detectable cells, less than 100, gives new possibilities for cellular MRI. Cell migration has shown to be an important issue concerning transplantation in PD.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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