Neuroscience 2002 Abstract
| Presentation Number: | 542.6 |
|---|---|
| Abstract Title: | Direct interactions between D<sub>2</sub>/D<sub>3</sub> dopamine receptors and AMPA glutamate receptors as demonstrated through co-immunoprecipitation analyses. |
| Authors: |
Kim, O. J.*1
; Han, J.1
; Sibley, D. R.1
1Molecular Neuropharmacology, NINDS/NIH, Bethesda, MD |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - G-Protein Linked Receptors -- Catecholamine receptors |
| Session: |
542. G-protein linked receptors: catecholamine receptors--interactors and signaling Poster |
| Presentation Time: | Tuesday, November 5, 2002 2:00 PM-3:00 PM |
| Location: | Hall A2-B3 D-19 |
| Keywords: | DOPAMINE RECEPTOR, AMPA RECEPTOR, GRIP, RECEPTOR BINDING |
We have previously shown (SFN 2001 Abstract #379.5) that D2/D3 dopamine receptors directly interact with GRIP, a PDZ domain-containing protein that interacts with GluR2/3 AMPA receptor subunits. GRIP functions as a scaffolding protein linking AMPA receptors and other signaling proteins into macromolecular complexes within postsynaptic membranes. Given this, and the fact that D2-like and AMPA receptors show cellular co-localization in the CNS (Synapse 26:400, 1997), we wondered if D2 or D3 receptors might form heterodimers with AMPA receptors, perhaps in a GRIP-facilitated fashion. As an initial test of this hypothesis, we co-expressed a FLAG-tagged D2 receptor or a c-myc-tagged D3 receptor with either GluR1, GluR2 or GluR4 AMPA receptor subunits in HEK293T cells. Radioligand binding assays confirmed the expression of the dopamine receptors while immunoblots confirmed the expression of the AMPA receptors. The cells were detergent-solubilized and the D2 or D3 receptors were immunoprecipitated with either anti-FLAG or anti-c-myc antisera, respectively. We found that the D2 and D3 receptors were co-immunoprecipitated with each AMPA receptor subunit – GluR1, GluR2 and GluR4. In contrast, no immunoprecipitation of any AMPA receptor subunit was observed from cells expressing the GluR subunits alone or with the GluR subunit co-expressed with either the FLAG or c-myc peptides only. Further experiments revealed that co-expression with AMPA receptors altered D2 or D3 radioligand binding activity in HEK293T cells. These results demonstrate that D2 and D3 dopamine receptors can directly interact AMPA receptors in a heterologous cellular expression system.
Supported by NIH
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
Copyright © 2002-2026 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
