Neuroscience 2005 Abstract
| Presentation Number: | 28.6 |
|---|---|
| Abstract Title: | Brief electrical stimulation promotes sensory neuron regeneration and intrinsic growth-associated gene expression. |
| Authors: |
Geremia, N. M.*1
; Gordon, T.2
; Al-Majed, A. A.4
; Brushart, T. M.3
; Verge, V. M. K.1
1Cameco MS Neurosci. Res. Center, Univ. of Saskatchewan, Saskatoon, Canada 2AB, 701 Queen St Rm 5800, S7K OM7, 3Canada, 701 Queen St Rm 5800, S7K OM7, 4Dept of Pharmacol, 701 Queen St Rm 5800, S7K OM7, |
| Primary Theme and Topics |
Development - Transplantation and Regeneration -- Regeneration: PNS |
| Secondary Theme and Topics | Development<br />- Axonal and Dendritic Development<br />-- Axon growth and guidance: Biological effects |
| Session: |
28. Regeneration: PNS I Poster |
| Presentation Time: | Saturday, November 12, 2005 2:00 PM-3:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # B61 |
| Keywords: | BDNF, DRG, plasticity, GAP-43 |
Electrical stimulation (stim), as brief as 1 hour, was found to enhance sensory neuron axonal regeneration. Stim for longer periods was less effective (Geremia et al., 2001 SfN Abstr 535.14). The beneficial effect of stim involves retrograde influences on the neuronal cell body, as TTX abolishes this response. The present study evaluates the effect of stim on the cell body response by evaluating its impact on expression of regeneration-associated genes (RAG). Using the femoral nerve model of transection and repair, the femoral nerve was stimulated proximal to repair at 20 Hz for 0 hour (sham), 1 hour or 3 hours. After a 2 day regeneration period, in situ hybridization and immunohistochemistry were performed on L3 DRG sections to detect changes in RAG mRNA expression (GAP-43 and Tα1 tubulin), BDNF, and trkB. Stim for 1 hour resulted in an upregulation of RAG, BDNF, and trkB when compared to sham. Stim for 3 hours resulted in decreased RAG and trkB expression, but increased BDNF-IR when compared to sham and 1 hour stim. Injured sensory neurons likely become desensitized in response to prolonged exposure of BDNF, as observed by the downregulation of trkB and RAG expression after 3 hour stim. Collectively, this likely contributes to the decreased axonal regenerative response observed at this time point. In conclusion, stim for 1 hour improves sensory axon regeneration and is associated with an enhanced regeneration-associated cell body response.
Supported by CIHR/Neurotrauma, U of S College of Med, and NIH.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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