Neuroscience 2004 Abstract
| Presentation Number: | 291.1 |
|---|---|
| Abstract Title: | Presynaptic kainate receptors are expressed in terminals of nociceptive primary afferents to the rat dorsal horn. |
| Authors: |
Lucifora, S.*1
; Lu, C.1
; Phend, K. D.1
; Willcockson, H. H.1
; Valtschanoff, J. G.1
; Rustioni, A.1
1Cell and Developmental Biol., Univ. of North Carolina, Chapel Hill, NC |
| Primary Theme and Topics |
Sensory Systems - Pain -- Spinal cord processing: anatomy and physiology |
| Session: |
291. Spinal Cord: Basic Mechanisms of Acute Pain Poster |
| Presentation Time: | Sunday, October 24, 2004 1:00 PM-2:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # O4 |
| Keywords: | GLUTAMATE RECEPTOR, PURINERGIC, PAIN, SPINAL CORD |
We have previously demonstrated presynaptic kainate receptors in the spinal cord of rats using immunostaining with a GluR 5/6/7 antibody and weak aldehyde fixation (Hwang et al., 2001). To further characterize primary afferents that express presynaptic kainate receptors, we studied with confocal microscopy double and triple immunofluorescent staining for GluR 5/6/7 and markers for glutamatergic and GABA-ergic terminals, and for various classes of nociceptive endings of primary afferent fibers. Throughout the L4-L5 segments of the spinal cord, puncta immunofluorescent for GluR 5/6/7 were densest in the superficial laminae of the dorsal horn and co-stained extensively with presynaptic markers synaptophysin and vesicular glutamate transporter (VGluT1), but not with a GABA-synthesizing enzyme (GAD-65), indicating that presynaptic kainate receptors are expressed predominantly by terminals of primary afferents (for EM evidence, see SFN abstract by Lu et al., 2004). Furthermore, GluR 5/6/7 colocalized in the inner part of lamina II with other presynaptic receptors characteristic of nociceptive afferents, although not to the same degree with each of them: highest degree of colocalization of presynaptic GluR 5/6/7 was with P2X3, the lowest (virtually negligible) was with CGRP. Colocalization of GluR 5/6/7 with VR1 was intermediate. These data suggest that presynaptic kainate receptors are expressed in terminals of non-peptidergic nociceptive primary afferents to the rat dorsal horn and may, thus, be selectively involved in the central mechanisms of nociception.
Supported by NIH grants #NS40885 and NS12440
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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