Neuroscience 2004 Abstract
| Presentation Number: | 232.18 |
|---|---|
| Abstract Title: | Glial and microglial reactions in a rodent cauda equina injury and repair model. |
| Authors: |
Ohlsson, M.*1
; Hoang, T. X.1
; Havton, L. A.1
1Dept of Neurol., UCLA, Los Angeles, CA |
| Primary Theme and Topics |
Motor Systems - Spinal Cord -- Damage and recovery |
| Session: |
232. Spinal Cord Injury: Inflammation Poster |
| Presentation Time: | Sunday, October 24, 2004 9:00 AM-10:00 AM |
| Location: | San Diego Convention Center - Hall A-H, Board # BBB22 |
| Keywords: | Spinal cord, Axotomy, Neuropil, Reinnervation |
In a recently developed rodent model for cauda equina/ conus medullaris forms of spinal cord injury, a lumbosacral ventral root avulsion (VRA) injury results in a progressive death of both autonomic and motor neurons (Hoang et al., JCN, 2003). An acute implantation of avulsed lumbosacral ventral roots into the spinal cord protects many of the axotomized neurons against cell death and promotes axonal regeneration by spinal cord neurons into the implanted graft (Hoang et al., SfN Abstract 76.15, 2003). The potential beneficial or detrimental role provided by the glial and microglial responses after a spinal cord injury remains controversial and their response patterns in this injury and repair model are unknown.
Here, we investigate the glial and microglial reactions in the rat conus medullaris to a lumbosacral VRA with and without subsequent implantation into the spinal cord. Control rats and rats with an L5-S2 VRA, with and without root implantation, are studied. Rats of the experimental series are studied for up to 3 months postoperatively. Control L6 and S1 spinal cord sections show a normal complement of Nissl stained and ChAT immunoreactive (IR) efferent neurons with a normal synaptophysin IR, a neuropil exhibiting a normal complement of GFAP IR, a few scattered Ox-42 IR and ramified microglial cells, and a near absence of ED-1 IR macrophages. Lumbosacral sections in the VRA injury series demonstrate a prominent loss of autonomic and motor neurons at 3 months postoperatively with a marked gliosis with numerous GFAP IR reactive astrocytes and glial processes in the intramedullary gray and white matters. The presence of many ED-1 IR profiles indicate a prominent recruitment of reactive microglia. Studies on the response by the glial and microglial markers following a lumbosacral VRA injury and subsequent acute implantation of avulsed ventral roots into the spinal cord is in progress.
Support contributed by: NIH/NINDS (NS42719); Paralysis Project of America; State of California-Roman Reed Bill.
Here, we investigate the glial and microglial reactions in the rat conus medullaris to a lumbosacral VRA with and without subsequent implantation into the spinal cord. Control rats and rats with an L5-S2 VRA, with and without root implantation, are studied. Rats of the experimental series are studied for up to 3 months postoperatively. Control L6 and S1 spinal cord sections show a normal complement of Nissl stained and ChAT immunoreactive (IR) efferent neurons with a normal synaptophysin IR, a neuropil exhibiting a normal complement of GFAP IR, a few scattered Ox-42 IR and ramified microglial cells, and a near absence of ED-1 IR macrophages. Lumbosacral sections in the VRA injury series demonstrate a prominent loss of autonomic and motor neurons at 3 months postoperatively with a marked gliosis with numerous GFAP IR reactive astrocytes and glial processes in the intramedullary gray and white matters. The presence of many ED-1 IR profiles indicate a prominent recruitment of reactive microglia. Studies on the response by the glial and microglial markers following a lumbosacral VRA injury and subsequent acute implantation of avulsed ventral roots into the spinal cord is in progress.
Support contributed by: NIH/NINDS (NS42719); Paralysis Project of America; State of California-Roman Reed Bill.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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