Neuroscience 2002 Abstract
| Presentation Number: | 221.2 |
|---|---|
| Abstract Title: | Serotonergic Neurons of the Midbrain Raphe Are Stimulated by Acidosis and Are Closely Associated with Arteries. |
| Authors: |
Severson, C. A.*1
; Wang, W.1
; Richerson, G. B.1,2,3
1Neurology, Yale University, New Haven, CT 2Physiology, Yale University, New Haven, CT 3VAMC, West Haven, CT |
| Primary Theme and Topics |
Autonomic, Limbic and Other Systems - Autonomic -- Respiratory regulation |
| Secondary Theme and Topics | Autonomic, Limbic and Other Systems<br />- Autonomic |
| Session: |
221. Autonomic: respiratory regulation I Slide |
| Presentation Time: | Monday, November 4, 2002 8:15 AM-8:30 AM |
| Location: | Room 206A |
| Keywords: | CHEMORECEPTOR, PH, RESPIRATION, AROUSAL |
Serotonergic neurons in the caudal raphé are chemosensitive, are intimately associated with arteries, and are putative central chemoreceptors. In the rostral raphé, serotonergic neurons are also stimulated by acidosis in both tissue culture and brain slices (Wang et al, SFN Abstracts, 2002). Here we examined whether rostral raphé neurons have the same relationship with arteries as those in the caudal raphé. Adult rats were transcardially perfused with formalin and arteries were filled with FITC labeled gelatin. Serotonergic neurons were immunolabeled with TpOH antibodies and visualized with confocal microscopy. Median raphé neurons were closely associated with midline penetrating arteries. The complex shape of the dorsal raphé paralleled the turns and branches of deep arteries in that region. We also noted that the midbrain is the only region of the rat CNS that is supplied by both the anterior and posterior circulations. To ensure that these observations were relevant to chemosensitive cells, acidosis-stimulated neurons were identified by patch clamp recordings from midbrain slices, injected with biocytin, stained and imaged. Acidosis-stimulated neurons were closely apposed to arteries in the dorsal raphé. These data highlight anatomical and physiological features common to the caudal and rostral raphé. In both cases, the proximity to arteries suggests that chemosensitive serotonergic neurons respond to arterial CO2 levels, allowing them to optimally monitor alveolar ventilation. These findings also implicate a putative CO2 chemosensory role for nuclei traditionally associated with the regulation of arousal and emotional state.
Supported by NIH & VAMC
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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