Neuroscience 2003 Abstract
| Presentation Number: | 102.7 |
|---|---|
| Abstract Title: | The combination of pre-treatment with dihydrolipoic acid and post-treatment with phenyl-butyl nitrone does not result in enhanced <I>in vitro</I> neuroprotection against an oxidative insult. |
| Authors: |
Koenig, M. L.*1
; Meyerhoff, J. L.1
1Div. of Neurosci., Walter Reed Army Inst. of Res., Silver Spring, MD |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia -- Neuroprotection and tolerance |
| Secondary Theme and Topics | Neurological and Psychiatric Conditions<br />- Neurotoxicity<br />-- Toxic metabolic effects and disorders |
| Session: |
102. Ischemia: Neuroprotection & Tolerance I Poster |
| Presentation Time: | Saturday, November 8, 2003 3:00 PM-4:00 PM |
| Location: | Morial Convention Center - Hall F-I, Board # RR11 |
| Keywords: | ANTIOXIDANT, OXIDATIVE STRESS, NEUROTOXICITY, CELL CULTURE |
One of the consequences of traumatic brain injury is an increase in the levels of reactive oxygen species (ROS). These highly reactive molecules contribute to the spread of secondary injury (surrounding the site of primary injury) by destabilizing neuronal membranes, denaturing proteins, and inducing strand breaks in nuclear DNA. In an earlier study we found that pre-treating cultures (4 h) with supplemental quantities of the endogenous antioxidant dihydrolipoic acid (DHLA) resulted in dose-dependent neuroprotection against H2O2-induced oxidative injury. Moreover, the addition of N-tert-butyl-alpha-phenyl nitrone (PBN) to the pre-treatment regimen enhanced the effect of DHLA (Neurotox. Res., in press). While DHLA requires pre-treatment to be neuroprotective, we found that PBN provides more effective neuroprotection when administered post-insult (SFN, 2002). The experiments described here evaluate the efficacy of pre-treating primary neuronal cultures with DHLA, exposing the neurons to an oxidative insult, and adding PBN to the DHLA pre-treated neurons immediately post-injury. Neuronal cultures were prepared from E-15 fetal rat forebrains, and neurotoxicity/neuroprotection was determined using the colorimetric MTT assay. Although a 4 h pre-treatment with DHLA resulted in dose-dependent neuroprotection, the post-injury addition of PBN (10 mM) to the DHLA pre-treated neurons did not result in a further enhancement of neuronal viability. Maximal neuroprotection in neurons pre-treated with DHLA (30 μM) was 38.54 ± 7.14% (n = 16). A subsequent addition of PBN immediately following the oxidative insult resulted in 39.02 ± 6.45% neuroprotection (n = 20). Additional experiments are being conducted to investigate further the apparent interaction between these two different classes of antioxidant.
Supported by US Army MRMC
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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