Neuroscience 2002 Abstract
| Presentation Number: | 151.18 |
|---|---|
| Abstract Title: | CA1 Long-Term Potentiation (LTP) Induced By Prolonged Theta Or High Frequency Stimulation Is Decreased With A Brief Application Of The Integrin Peptide GRGDSP During Induction. |
| Authors: |
Hernandez, R. V.*1
; Navarro, M. M.1
; Garza, J. M.1
; Martinez, J. L.1
; LeBaron, R. G.1
1Dept of Biology and the Cajal Neuroscience Research Center, University of Texas, San Antonio, TX |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Synaptic Plasticity -- Long-term potentiation (LTP) |
| Session: |
151. Synaptic plasticity: long-term potentiation III Poster |
| Presentation Time: | Sunday, November 3, 2002 2:00 PM-3:00 PM |
| Location: | Hall A2-B3 E-61 |
| Keywords: | Hippocampus, Rat, Extracellular Matrix, Cell Adhesion Molecules |
Magnitude and chemistry of LTP may depend on whether the induction paradigm is a theta burst (TBS) or a high frequency (HFS) stimulation pattern. TBS mimics endogenous neural activity but many studies also use HFS to induce LTP. We previously reported that a systematic comparison of TBS and HFS in hippocampal slices revealed that the magnitude of LTP was determined more by the total number of pulses rather than stimulation pattern (SFN Abstract, Vol. 27, 372.4, 2001). Two hundred and three hundred pulses produced significantly greater LTP in both paradigms when compared to the forty or one hundred pulse paradigms. We are currently investigating whether integrins are differentially involved in robust LTP resulting from the greater number of pulses. In CA1, TBS of 200 pulses and a 15 min bath application of GRGDSP peptide during induction significantly reduces field LTP at 250 μM (70.3% ± 5.0, n = 4) and 500 μM (49.6% ± 2.7, n = 3), when compared to ACSF controls (123.8% ± 18.8, n = 3; ANOVA, p <.05). HFS with 200 pulses and 500 μM GRGDSP also substantially reduces LTP (46% ± 31, n = 2) versus controls (87% ± 27, n = 3). Both paradigms retain significant LTP one hour after tetanus. These preliminary data indicate that integrins likely contribute to synaptic plasticity induced by either stimulation paradigm. Residual LTP could suggest that integrins recognizing the GRGDSP sequence are not sufficient for LTP expression or maintenance.
Supported by NINDS Grant NS39404-02S1 (RVH), NS39409 (RGL and JLM) and the Ewing Halsell Endowment (JLM).
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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