Neuroscience 2001 Abstract
| Presentation Number: | 922.3 |
|---|---|
| Abstract Title: | Development of complex activity patterns in cortical networks cultured on multi-electrode arrays. |
| Authors: |
Wagenaar, D. A.*1
; DeMarse, T. B.2
; Potter, S. M.2
; Pine, J.1
1Physics, Caltech, Pasadena, CA 2Biology, Caltech, Pasadena, CA |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Synaptic Plasticity -- Other |
| Secondary Theme and Topics | Development<br />- Synaptogenesis and Activity-Dependent Development<br />-- Activity-dependent development and plasticity |
| Session: |
922. Synaptic plasticity: other--network activity and modeling Poster |
| Presentation Time: | Thursday, November 15, 2001 10:00 AM-11:00 AM |
| Location: | Exhibit Hall G-27 |
| Keywords: | animat, in vitro, MEA, network dynamics |
Cultured cortical networks exhibit a tremendous range of firing behavior. We are looking for regularities in their firing patterns, which we plan to use to control a simulated animal (the Neurally Controlled Animat; Potter group, SFN 2000 #467.20). To this end, neurons and glia from E18 rat cortex were dissociated and densely plated on planar MEAs (multi-electrode arrays) with 60 electrodes. We recorded daily from each dish for 30 consecutive days, starting one day after plating. Recurring dynamic patterns were observed on many timescales, from less than 100 ms through minutes.
Single-cell action potentials were observed from the second day in vitro. Dish-wide bursts occurred as early as the fifth day. As the cultures matured, bursts became increasingly frequent. Isolated spikes, while increasing in absolute numbers, became an ever smaller part of the dishes' activity patterns. The dishes produced global bursts between one and 30 times per minute. Often, periodicity was maintained with few interruptions for several minutes.
Most global bursts were found to be immediately preceded (within 50 ms) by elevated activity of neurons near only one or a few electrodes. These initiator sets changed as the cultures developed. Recordings that showed the highest global burst frequencies, often exhibited abrupt switching between very low (3/min) and very high (upto 60/min) burst frequencies, in cycles of up to 3 minutes.
These observations will provide the basis for a study of the effects of chronic (continuous) electrical stimulation on cortical networks developing in vitro.
Single-cell action potentials were observed from the second day in vitro. Dish-wide bursts occurred as early as the fifth day. As the cultures matured, bursts became increasingly frequent. Isolated spikes, while increasing in absolute numbers, became an ever smaller part of the dishes' activity patterns. The dishes produced global bursts between one and 30 times per minute. Often, periodicity was maintained with few interruptions for several minutes.
Most global bursts were found to be immediately preceded (within 50 ms) by elevated activity of neurons near only one or a few electrodes. These initiator sets changed as the cultures developed. Recordings that showed the highest global burst frequencies, often exhibited abrupt switching between very low (3/min) and very high (upto 60/min) burst frequencies, in cycles of up to 3 minutes.
These observations will provide the basis for a study of the effects of chronic (continuous) electrical stimulation on cortical networks developing in vitro.
Supported by This work is supported by grant #R01NS38628 from NIH/NINDS, and by the Burroughs-Wellcome/Caltech CMB fund.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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